Department of Botany, Faculty of Science, Khartoum University, Khartoum, Sudan.
Int J Phytoremediation. 2023;25(3):314-321. doi: 10.1080/15226514.2022.2083575. Epub 2022 Jun 10.
Phytoremediation is a new technology for cleaning contaminated soil with crude oil. Oil pollution is a serious problem worldwide; the aim of this study was to use the plant for Phytoremediation. Leguminous plant Tausch was tested for it is efficiency to remediate soil polluted with 0.5, 1, 1.5 and 2% (w/w) crude oil and it is the ability to enhance the activity of soil microorganisms. Plant parameters, degradation percentage, Total Petroleum Hydrocarbon and bacterial count were measured. Results showed that the concentration of the crude oil did not affect plant growth indicating the significant success of the Phytoremediation process. Shoot length and shoot and root weights have not been negatively affected by oil, compared to the control plant, up to a concentration of 1.5% for up to six months. Crude oil degradation percentages were found to be in the range of 49-79%. The highest degradation percentage was recorded for the soil collected from underneath Tausch seedlings (79%). A total of 81 different hydrocarbons were detected in soil samples at zero time and most of them were found to be of long (≥30 carbon atoms) and moderate (10-29 C) hydrocarbon chains. Fraction analyses were conducted in plant Tausch seedlings at intervals of 60, 120 and 180 days of incubation, six different hydrocarbons were detected. The most abundant hydrocarbon detected were Heneicosane (21 C), Tetracosane (24 C) and Octacosane (28 C). The viable microorganism's count in oil-contaminated soil at any of the concentrations tested was significantly ( ≤ 0.01) higher than that in oil-free soil at any sampling interval. High efficiency of biodegradation was achieved using Tausch indicating the unique mechanism of this plant in remediating contaminated soil with crude oil.
植物修复是一种利用植物修复受原油污染土壤的新技术。石油污染是一个全球性的严重问题；本研究的目的是利用植物进行植物修复。选择豆科植物 Tausch 进行测试，以评估其修复 0.5%、1%、1.5%和 2%（w/w）原油污染土壤的效率及其增强土壤微生物活性的能力。测量了植物参数、降解百分比、总石油烃和细菌计数。结果表明，原油浓度不影响植物生长，表明植物修复过程取得了显著成功。与对照植物相比，在长达六个月的时间内，油的浓度高达 1.5%，对茎长和茎重及根重没有负面影响。发现原油的降解百分比在 49-79%范围内。从 Tausch 幼苗下采集的土壤的最高降解百分比为 79%。在零时间，从土壤样本中检测到 81 种不同的碳氢化合物，其中大多数是长链（≥30 个碳原子）和中链（10-29 C）碳氢化合物。在 60、120 和 180 天的孵育间隔内对 Tausch 幼苗中的植物进行了馏分分析，检测到六种不同的碳氢化合物。检测到的最丰富的碳氢化合物是heneicosane（21 C）、tetracosane（24 C）和octacosane（28 C）。在任何测试浓度下，受油污染土壤中的可培养微生物计数均明显（≤0.01）高于任何采样间隔下无油土壤中的计数。Tausch 实现了高效的生物降解，表明该植物在修复受原油污染的土壤方面具有独特的机制。
