Xie Juan-Ping, Xiang Ji-Ming, Zhu Zhong-Liang
Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi'an 710069, China Department of Chemistry and Chemical Engineering, Ankang University, Ankang, 725000, China.
Department of Chemistry and Chemical Engineering, Ankang University, Ankang, 725000, China.
J Chromatogr Sci. 2016 Apr;54(4):664-9. doi: 10.1093/chromsci/bmw007. Epub 2016 Feb 9.
A simple, accurate and reproducible method which is based on the capillary electrophoresis, coupled with solid-phase extraction, has been developed for simultaneous determination of multiple 8-prenylflavones from Chinese Herba Epimedii. In this study, the author has mainly illustrated the experimental process and research results of five major components including epimedin C, icariin, diphylloside A, epimedoside A and icarisoside A that have been extracted and identified from Herba Epimedii for the first time. Experimental conditions have been optimized to achieve the best separation efficiency for the following factors: the buffer pH, buffer concentration and applied voltage. The experiment can be conducted through two separable stages: the first stage is to obtain the crude extracts through the solid-phase extraction; and the second stage is to further separate five major components by using the capillary electrophoresis. The separation of the five components and the analysis of the experiment are relatively fast and can be completed within 20 min. The concentration ranges of the construction of standard curves of five major 8-prenylflavones are 32.0-395.0, 23.4-292.0, 42.1-526.0, 18.8-233.5 and 29.7-371.0 µg mL(-1) respectively, which have showed acceptable linearity with a correlation coefficient, r ≥ 0.999. The coefficient varies within 2.0% for both intra- and inter-days tests. The recoveries of five components range from 92.3 to 104.1%. The relative standard deviations of recoveries of five components range from 1.2 and 2.8%. This new method will facilitate the extraction and expedite the determination of medical components from Herba Epimedii.
基于毛细管电泳结合固相萃取，开发了一种简单、准确且可重复的方法，用于同时测定淫羊藿中多种8 - 异戊烯基黄酮。在本研究中，作者主要阐述了首次从淫羊藿中提取并鉴定出的包括朝藿定C、淫羊藿苷、二叶苷A、淫羊藿新苷A和淫羊藿糖苷A这五种主要成分的实验过程和研究结果。对实验条件进行了优化，以实现以下因素的最佳分离效率：缓冲液pH值、缓冲液浓度和施加电压。该实验可通过两个可分离的阶段进行：第一阶段是通过固相萃取获得粗提物；第二阶段是利用毛细管电泳进一步分离五种主要成分。这五种成分的分离和实验分析相对较快，可在20分钟内完成。五种主要8 - 异戊烯基黄酮标准曲线构建的浓度范围分别为32.0 - 395.0、23.4 - 292.0、42.1 - 526.0、18.8 - 233.5和29.7 - 371.0 μg mL(-1)，其线性关系良好，相关系数r≥0.999。日内和日间测试的系数变化均在2.0%以内。五种成分的回收率在92.3%至104.1%之间。五种成分回收率的相对标准偏差在1.2%至2.8%之间。这种新方法将有助于淫羊藿药用成分的提取并加快其测定。
