Vitta Apichat, Yimthin Thatcha, Fukruksa Chamaiporn, Wongpeera Wichuda, Yotpanya Waranan, Polseela Raxsina, Thanwisai Aunchalee
Southeast Asian J Trop Med Public Health. 2015 Jul;46(4):564-73.
Entomopathogenic nematodes (EPNs) are used successfully for biological control of subterranean larval pests leading to reduced environmental contamination if chemical control measures are employed. Their diversity and distribution in Thailand are unclear, so the present study sought to obtain a better understanding these EPN populations in the lower northern region of Thailand. We collected 930 soil samples from 186 sites of Kamphaeng Phet, Nakhon Sawan, Phetchabun, Phichit, Phitsanulok, Sukhothai, Tak, Uthai Thani, and Uttaradit Provinces, Thailand from December 2011 to November 2012. Galleria mellonella was used as host for isolating and propagating EPNs. Seventy soil samples (7.5%) yielded EPNs of two genera, Steinernema (3.0%) and Heterorhabditis (4.5%). The majority of the isolated EPNs were found in loam at 26°C-33°C and pH values of 5.0-7.0. Molecular identification from partial 28S rDNA sequences revealed S. websteri, isolated from soil samples from Nakhon Sawan and Uthai Thani. Phylogenetic analysis of these EPNs showed they are closely related to S. websteri JC1032. The identification that S. websteri was the predominant EPN should enable its application for biological control in the local prevailing soil conditions.
昆虫病原线虫（EPNs）已成功用于地下幼虫害虫的生物防治，若采用化学防治措施则会导致环境污染加剧。其在泰国的多样性和分布情况尚不清楚，因此本研究旨在更好地了解泰国北部低地地区的这些EPN种群。2011年12月至2012年11月，我们从泰国北榄坡府、那空沙旺府、碧差汶府、彭世洛府、素可泰府、达府、乌泰他尼府和乌塔拉迪特府的186个地点采集了930份土壤样本。大蜡螟被用作分离和繁殖EPNs的宿主。70份土壤样本（7.5%）分离出了两个属的EPNs，即斯氏线虫属（3.0%）和异小杆线虫属（4.5%）。大多数分离出的EPNs发现于壤土中，温度为26°C至33°C，pH值为5.0至7.0。通过对部分28S rDNA序列进行分子鉴定，从那空沙旺府和乌泰他尼府的土壤样本中分离出了韦氏斯氏线虫。这些EPNs的系统发育分析表明它们与韦氏斯氏线虫JC1032密切相关。韦氏斯氏线虫是主要EPNs这一鉴定结果应能使其应用于当地主要土壤条件下的生物防治。
