Departamento de Proteção Vegetal, Universidade Estadual Paulista (UNESP/FCA), Rua José Barbosa de Barros, 1780, CEP 18610-307, Botucatu, São Paulo, Brasil.
Laboratório de Nematologia (CEIB), Instituto Biológico, Alameda dos Vidoeiros, 1097, CEP 13101-680, Campinas, São Paulo, Brasil.
Sci Rep. 2017 Apr 6;7:45254. doi: 10.1038/srep45254.
Entomopathogenic nematodes (EPNs) (Steinernematidae and Heterorhabditidae) can control pests due to the mutualistic association with bacteria that kill the host by septicemia and make the environment favorable for EPNs development and reproduction. The diversity of EPNs in Brazilian soils requires further study. The identification of EPNs, adapted to environmental and climatic conditions of cultivated areas is important for sustainable pest suppression in integrated management programs in agricultural areas of Brazil. The objective was to identify EPNs isolated from agricultural soils with annual, fruit and forest crops in Brazil. Soil samples were collected and stored in 250 ml glass vials. The nematodes were isolated from these samples with live bait traps ([Galleria mellonella L. (Lepidoptera: Pyralidae) larvae]. Infective juveniles were collected with White traps and identified by DNA barcoding procedures by sequencing the D2/D3 expansion of the 28S rDNA region by PCR. EPNs identified in agricultural areas in Brazil were Heterorhabditis amazonensis, Metarhabditis rainai, Oscheios tipulae and Steinernema rarum. These species should be considered pest biocontrol agents in Brazilian agricultural areas.
昆虫病原线虫（EPNs）（Steinernematidae 和 Heterorhabditidae）可以通过与细菌的共生关系来控制害虫，这些细菌通过败血症杀死宿主，并使环境有利于 EPNs 的发育和繁殖。巴西土壤中 EPNs 的多样性需要进一步研究。鉴定适应巴西耕作区环境和气候条件的 EPNs 对于在农业区综合管理计划中实现可持续的害虫防治至关重要。本研究的目的是从巴西种植一年生作物、水果和森林的农田土壤中分离鉴定 EPNs。采集土壤样本并储存在 250ml 玻璃小瓶中。用活诱饵陷阱（[Galleria mellonella L.（鳞翅目：Pyralidae）幼虫]）从这些样本中分离出线虫。用 White 陷阱收集感染性幼虫，并通过 PCR 扩增 28S rDNA 区的 D2/D3 区进行 DNA 条码分析来鉴定。在巴西农业区鉴定出的 EPNs 为 Heterorhabditis amazonensis、Metarhabditis rainai、Oscheios tipulae 和 Steinernema rarum。这些物种应被视为巴西农业区的害虫生物防治剂。
