Lidan Chen, Linhai Li, Yang Liao, Zhaohui Sun, Xiaoyan Huang, Yuling Shi
Graduate School of Southern Medical University, Guangzhou, Guangdong 510515, PR China; Department of Laboratory Medicine, Guangzhou General Hospital of Guangzhou Military Command of PLA, Guangzhou, Guangdong 510010, PR China.
Department of Laboratory Medicine, Guangzhou General Hospital of Guangzhou Military Command of PLA, Guangzhou, Guangdong 510010, PR China.
Diagn Microbiol Infect Dis. 2016 Apr;84(4):361-5. doi: 10.1016/j.diagmicrobio.2015.12.015. Epub 2015 Dec 23.
This study aimed to investigate the molecular characteristics and antimicrobial susceptibility of Clostridium difficile clinical isolates in Guangzhou, China. One hundred twenty isolates were collected from Guangzhou General Hospital at the Guangzhou Military Command in China from March 2014 to April 2015, and 9 isolates were identified as tcdA-negative/tcdB-positive (A(-)B(+)) strains. Results showed that all of the strains were confirmed to be ST37 and 0 single nucleotide variants (SNVs) were found in the PaLoc region, and >60 SNVs were identified throughout the whole genome sequence. The results show the diversity of the antibiotic and gene mutations present in these strains. All of the A(-)B(+) isolates were highly resistant to clindamycin and erythromycin; showed an average sensitivity to fluoroquinolones; and maintained a high susceptibility to metronidazole, vancomycin, and tigecycline.
本研究旨在调查中国广州艰难梭菌临床分离株的分子特征及抗菌药敏情况。2014年3月至2015年4月期间，从中国广州军区广州总医院收集了120株分离株，其中9株被鉴定为tcdA阴性/tcdB阳性（A(-)B(+)）菌株。结果显示，所有菌株均被确认为ST37型，在致病岛（PaLoc）区域未发现单核苷酸变异（SNV），但在整个基因组序列中鉴定出>60个SNV。结果表明这些菌株中存在抗生素和基因突变的多样性。所有A(-)B(+)分离株对克林霉素和红霉素高度耐药；对氟喹诺酮类药物表现出平均敏感性；对甲硝唑、万古霉素和替加环素保持高度敏感。
