Afzal M B S, Shad S A
Dept of Entomology, Fac of Agricultural Sciences and Technology, Bahauddin Zakariya Univ Multan, Multan, Pakistan.
Neotrop Entomol. 2016 Jun;45(3):310-9. doi: 10.1007/s13744-016-0370-5. Epub 2016 Feb 11.
Cotton mealybug Phenacoccus solenopsis (Tinsley) (Homoptera: Pseudococcidae) is a sucking pest of worldwide importance causing huge losses by feeding upon cotton in various parts of the world. Because of the importance of this pest, this research was carried out to select emamectin resistance in P. solenopsis in the laboratory to study cross-resistance, stability, realized heritability, and fitness cost of emamectin resistance. After selection from third generation (G3) to G6, P. solenopsis developed very high emamectin resistance (159.24-fold) when compared to a susceptible unselected population (Unsel pop). Population selected to emamectin benzoate conferred moderate (45.81-fold), low (14.06-fold), and no cross-resistance with abamectin, cypermethrin, and profenofos, respectively compared to the Unsel pop. A significant decline in emamectin resistance was observed in the resistant population when not exposed to emamectin from G7 to G13. The estimated realized heritability (h (2)) for emamectin resistance was 0.84. A high fitness cost was associated with emamectin resistance in P. solenopsis. Results of this study may be helpful in devising insecticide resistance management strategies for P. solenopsis.
棉粉蚧，即扶桑绵粉蚧（Tinsley）（同翅目：粉蚧科），是一种具有全球重要性的刺吸式害虫，通过吸食世界各地不同地区的棉花，造成了巨大损失。鉴于该害虫的重要性，开展了本研究，旨在在实验室中筛选扶桑绵粉蚧对甲氨基阿维菌素苯甲酸盐的抗性，以研究其交互抗性、稳定性、现实遗传力以及甲氨基阿维菌素苯甲酸盐抗性的适合度代价。从第三代（G3）到第六代进行筛选后，与未筛选的敏感种群（未筛选种群）相比，扶桑绵粉蚧对甲氨基阿维菌素苯甲酸盐产生了非常高的抗性（159.24倍）。与未筛选种群相比，筛选得到的对甲氨基阿维菌素苯甲酸盐的种群，对阿维菌素、氯氰菊酯和丙溴磷分别表现出中等抗性（45.81倍）、低抗性（14.06倍）且无交互抗性。当从第七代到第十三代不接触甲氨基阿维菌素苯甲酸盐时，抗性种群中观察到甲氨基阿维菌素苯甲酸盐抗性显著下降。甲氨基阿维菌素苯甲酸盐抗性的估计现实遗传力（h²）为0.84。扶桑绵粉蚧的甲氨基阿维菌素苯甲酸盐抗性与高适合度代价相关。本研究结果可能有助于制定扶桑绵粉蚧的抗药性治理策略。
