Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
Department of Electrical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.
Biosens Bioelectron. 2017 Mar 15;89(Pt 1):598-605. doi: 10.1016/j.bios.2016.01.077. Epub 2016 Jan 29.
Early diagnosis of prostate cancer (PCa) is critical for the prevention of metastasis and for early treatment; therefore, a simple and accurate device must be developed for this purpose. In this study, we reported a novel fabrication method for producing a dual-modality biosensor that can simultaneously detect vascular endothelial growth factor (VEGF) and prostate-specific antigen (PSA) in human serum for early diagnosis of PCa. This biosensor was constructed by coating graphene oxide/ssDNA (GO-ssDNA) on an Au-electrode for VEGF detection, and incorporated with poly--lactide nanoparticles (PLLA NPs) for signal amplification and PSA detection. The results showed that this biosensor has wide liner detection ranges (0.05-100ng/mL for VEGF and 1-100ng/mL for PSA), as well as high levels of sensitivity and selectivity (i.e., resisting interference from external factors, such as glucose, ascorbic acid human serum protein, immunoglobulin G, and immunoglobulin M), and demonstrated a high correlation with an enzyme-linked immunosorbent assay for sample detection in patients. Therefore, this biosensor could be utilized for early clinical diagnosis of PCa in the future.
早期诊断前列腺癌（PCa）对于预防转移和早期治疗至关重要；因此，必须为此目的开发一种简单而准确的设备。在这项研究中，我们报告了一种用于制造双模态生物传感器的新方法，该传感器可以同时检测人血清中的血管内皮生长因子（VEGF）和前列腺特异性抗原（PSA），用于早期诊断 PCa。该生物传感器通过在 Au 电极上涂覆氧化石墨烯/ssDNA（GO-ssDNA）来检测 VEGF，并结合聚乳酸纳米粒子（PLLA NPs）来进行信号放大和 PSA 检测。结果表明，该生物传感器具有较宽的线性检测范围（VEGF 为 0.05-100ng/mL，PSA 为 1-100ng/mL），并且具有较高的灵敏度和选择性（即，能抵抗葡萄糖、抗坏血酸、人血清蛋白、免疫球蛋白 G 和免疫球蛋白 M 等外部因素的干扰），并且与酶联免疫吸附试验（ELISA）具有高度相关性，可用于患者样本检测的早期临床诊断。因此，该生物传感器未来可用于 PCa 的早期临床诊断。
