Department of Internal Medicine, Division of Allergy, Immunology and Rheumatology, Chang Gung Memorial Hospital at Keelung, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC.
Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, Chang Gung Memorial Hospital at Keelung, Taoyuan, Taiwan, ROC.
J Microbiol Immunol Infect. 2017 Dec;50(6):857-862. doi: 10.1016/j.jmii.2015.12.010. Epub 2016 Jan 12.
Raynaud's phenomenon (RP) is a microvascular disorder characterized by episodic peripheral vasospasm and ischemia and is commonly found in patients with autoimmune diseases (AID). The vasomotor homoeostasis and endothelial cells damage are involved in RP. Endothelial microparticles (EMPs) may act as a biomarker for endothelial damage. The aim of this study is to investigate the correlation between the levels of microparticles (MPs) and microvasculopathy in AID with RP.
Thirty-seven patients with AID and RP (RP group) and 27 patients with AID but without RP (non-RP group) were enrolled. The microvasculopathy score of RP was graded by nailfold capillary microscopy. The plasma levels of MPs were measured by flow cytometry utilizing specific labels for endothelial MPs (CD105 and CD144) and annexin V staining for phosphatidylserine bearing-MPs (annexin V+MPs).
The levels of circulating EMPs (CD105+ p = 0.005, CD144+ p = 0.004), and the annexin V+ MPs (p < 0.001) were significantly elevated in the RP group compared with the non-RP group. Moreover, the high microvasculopathy scores were closely related with annexinV+ MPs levels in the RP group (p = 0.041).
Levels of circulating EMPs and annexin V+ MPs are elevated in AID patients with RP indicate the endothelial damage and endothelial dysfunctions. In addition, levels of annexin V+ MPs can predict the severity of microvasculopathy in AID with RP.
雷诺现象（RP）是一种以间歇性周围血管痉挛和缺血为特征的微血管疾病，常见于自身免疫性疾病（AID）患者。血管舒缩稳态和内皮细胞损伤与 RP 有关。内皮细胞微颗粒（EMP）可能作为内皮损伤的生物标志物。本研究旨在探讨 AID 伴 RP 患者中微颗粒（MP）水平与微血管病变的相关性。
纳入 37 例 AID 伴 RP（RP 组）和 27 例 AID 但无 RP（非 RP 组）患者。通过甲襞毛细血管显微镜对 RP 的微血管病变评分进行分级。采用流式细胞术利用内皮细胞 EMP 的特异性标记物（CD105 和 CD144）和载有磷脂酰丝氨酸的膜联蛋白 V 染色（annexin V+ MPs）测量血浆 MPs 水平。
与非 RP 组相比，循环 EMPs（CD105+，p=0.005，CD144+，p=0.004）和 annexin V+ MPs（p<0.001）水平在 RP 组中显著升高。此外，在 RP 组中，高微血管病变评分与 annexin V+ MPs 水平密切相关（p=0.041）。
AID 伴 RP 患者循环 EMPs 和 annexin V+ MPs 水平升高表明内皮损伤和内皮功能障碍。此外，annexin V+ MPs 水平可预测 AID 伴 RP 患者微血管病变的严重程度。
