Rani Pooja, Mukherjee Udita, Verma Helianthous, Kamra Komal, Lal Rup
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi- 110007, India.
Ciliate Biology Laboratory, SGTB Khalsa College, University of Delhi, Delhi- 110007, India.
Int J Syst Evol Microbiol. 2016 Apr;66(4):1851-1856. doi: 10.1099/ijsem.0.000956. Epub 2016 Feb 10.
A Gram-stain-negative, aerobic, rod-shaped, non-spore-forming, yellow pigmented bacterial strain (UM1T) was isolated from the hexachlorocyclohexane (HCH)-contaminated dumpsite located at Ummari village in Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UM1T belongs to the genus Luteimonas with Luteimonas aestuarii B9T as the closest neighbour (97.2% 16S rRNA gene sequence similarity). The DNA G+C content of strain UM1T was 64.3 mol%. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). Main fatty acids were iso-C15:0, iso-C11:0, iso-C11:0 3-OH, iso-C17:0 and summed feature 9 (C16:0 10-methyl and/or iso-C17:1ω9c). Ubiquinone (Q-8) was the only respiratory quinone. Spermidine was detected as the major polyamine. The DNA-DNA relatedness value of strain UM1T with respect to its closest neighbour Luteimonas aestuarii B9T was well below 70 % (∼49%). Thus, data obtained from phylogenetic analysis, DNA-DNA hybridization, and chemotaxonomical and biochemical analyses supports classification of strain UM1T as representative of a novel species of the genus Luteimonas, for which the name Luteimonas tolerans sp. nov. is proposed. The type strain is UM1T (=DSM 28473T=MCC 2572T=KCTC 42936T).
从印度勒克瑙乌马里村受六氯环己烷（HCH）污染的垃圾场中分离出一株革兰氏染色阴性、需氧、杆状、不形成芽孢、产黄色色素的细菌菌株（UM1T）。基于16S rRNA基因序列的系统发育分析表明，菌株UM1T属于Luteimonas属，与Luteimonas aestuarii B9T关系最为密切（16S rRNA基因序列相似性为97.2%）。菌株UM1T的DNA G+C含量为64.3 mol%。主要极性脂为双磷脂酰甘油（DPG）、磷脂酰乙醇胺（PE）和磷脂酰甘油（PG）。主要脂肪酸为异C15:0、异C11:0、异C11:0 3-OH、异C17:0和总和特征9（C16:0 10-甲基和/或异C17:1ω9c）。泛醌（Q-8）是唯一的呼吸醌。检测到亚精胺为主要多胺。菌株UM1T与其最密切相关的Luteimonas aestuarii B9T的DNA-DNA杂交相关值远低于70%（约49%）。因此，从系统发育分析、DNA-DNA杂交以及化学分类和生化分析获得的数据支持将菌株UM1T分类为Luteimonas属一个新物种的代表，为此提出Luteimonas tolerans sp. nov.这个名称。模式菌株为UM1T（=DSM 28473T=MCC 2572T=KCTC 42936T）。
