State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Int J Syst Evol Microbiol. 2020 May;70(5):3462-3467. doi: 10.1099/ijsem.0.004197.
A Gram-stain-negative, rod-shaped bacterium, strain H23, was isolated from farmland soil sampled in Enshi City, Hubei Province, PR China. The isolate grew optimally at 28-32 °C, pH 8.0 and with 0.5 % (w/v) NaCl. Based on the results of 16S rRNA gene sequence and phylogenetic analyses, strain H23 belonged to the genus with the highest degree of 16S rRNA gene sequence similarity to Y4 (97.41 %). The DNA G+C content was 65.88 mol%. The average nucleotide identity and the Genome-to-Genome Distance Calculator results also showed low relatedness (below 95 and 70 %, respectively) between strain H23 and type strains in the genus . Ubiquinone-8 was the predominant quinone. The major fatty acids were iso-C, iso-C, iso-C and iso-C ω9. Polar lipids were dominated by diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unidentified phospholipids. Low digital DNA-DNA hybridization values, as well as physiological and biochemical differences, such as no casein hydrolysis, being catalase-negative, and tesing positive for cystine arylamidase, α-chymotrypsin and -acetyl-β-glucosaminidase, could distinguish strain H23 from its closely related species. Strain H23 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed, with strain H23 (=CCTCC AB 2019255=KCTC 72593) as the type strain.
一株革兰氏阴性、杆状细菌，菌株 H23，从中国湖北省恩施市农田土壤中分离得到。该分离株在 28-32°C、pH8.0 和 0.5%（w/v）NaCl 下生长最佳。基于 16S rRNA 基因序列和系统发育分析的结果，菌株 H23属于 属，与 Y4（97.41%）的 16S rRNA 基因序列相似度最高。DNA G+C 含量为 65.88mol%。平均核苷酸同一性和基因组到基因组距离计算器的结果也表明，菌株 H23与该属的模式菌株之间的相关性较低（低于 95%和 70%）。泛醌-8 是主要的醌。主要脂肪酸为 iso-C、iso-C、iso-C 和 iso-C ω9。极性脂主要由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和未鉴定的磷脂组成。低数字 DNA-DNA 杂交值以及生理生化差异，如无酪蛋白水解、过氧化氢酶阴性以及对胱氨酸芳基酰胺酶、α-糜蛋白酶和 -乙酰-β-葡萄糖胺酶呈阳性反应，可将菌株 H23 与密切相关的种区分开来。菌株 H23 被认为代表了属中的一个新种，建议将其命名为 sp. nov.，菌株 H23（=CCTCC AB 2019255=KCTC 72593）作为模式菌株。
