Jiang Nan, Wei Kai, Chen Lijun, Chen Rui
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P.R. China.
Tianjin Institute of Agricultural Quality Standard and Testing Technology, Tianjin Academy of Agricultural Sciences, Tianjin 300381, P.R. China.
J Microbiol Biotechnol. 2016 May 28;26(5):867-75. doi: 10.4014/jmb.1511.11012.
Archaea substantially contribute to global geochemical cycling and energy cycling and are impacted by land-use change. However, the response of archaeal communities to a change from upland field to paddy field has been poorly characterized. Here, soil samples were collected at two depths (0-20 cm and 20-40 cm) from one upland field and six paddy fields that were established on former upland fields at different times (1, 5, 10, 20, 30, and 40 years before the study). Barcoded pyrosequencing was employed to assess the archaeal communities from the samples at taxonomic resolutions from phylum to genus levels. The total archaeal operational taxonomic unit (OTU) richness showed a significant positive correlation with the land-use change duration. Two phyla, Euryarchaeota and Crenarchaeota, were recorded throughout the study. Both the relative abundance and OTU richness of Euryarchaeota increased at both depths but increased more steadily at the subsurface rather than at the surface. However, these data of Crenarchaeota were the opposite. Additionally, the archaeal composition exhibited a significant relationship with C/N ratios, total phosphorus, soil pH, Olsen phosphorus, and the land-use change duration at several taxonomic resolutions. Our results emphasize that after a change from upland fields to paddy fields, the archaeal diversity and composition changed, and the duration is an important factor in addition to the soil chemical properties.
古菌对全球地球化学循环和能量循环有重大贡献，并受到土地利用变化的影响。然而，古菌群落对从旱地向水田转变的响应特征尚不明确。在此，从一块旱地以及在不同时间（研究前1、5、10、20、30和40年）由前旱地改造而成的六块水田中，采集了两个深度（0 - 20厘米和20 - 40厘米）的土壤样本。采用条形码焦磷酸测序技术，从门到属水平的分类分辨率评估样本中的古菌群落。古菌的总操作分类单元（OTU）丰富度与土地利用变化持续时间呈显著正相关。在整个研究过程中记录到了广古菌门和泉古菌门两个菌门。广古菌门在两个深度的相对丰度和OTU丰富度均增加，但在地下层比表层增加得更稳定。然而，泉古菌门的数据情况则相反。此外，在几个分类分辨率水平上，古菌组成与碳氮比、总磷、土壤pH值、有效磷以及土地利用变化持续时间呈现出显著关系。我们的研究结果强调，从旱地转变为水田后，古菌的多样性和组成发生了变化，除土壤化学性质外，持续时间也是一个重要因素。
