Zhang Xiaochen, Zhang Zhiyuan, Shen Gang, Zhao Jun
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Department of Orthodontics, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Int J Nanomedicine. 2016 Jan 25;11:429-39. doi: 10.2147/IJN.S93177. eCollection 2016.
To enhance biocompatibility and osseointegration between titanium implants and surrounding bone tissue, numerous efforts have been made to modify the surface topography and composition of Ti implants. In this paper, Lenti-BMP-2-loaded TiO2 nanotube coatings were fabricated by lyophilization in the presence of trehalose to functionalize the surface. We characterized TiO2 nanotube layers in terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration in vivo. The anodized TiO2 nanotube surfaces exhibited an amorphous glassy matrix perpendicular to the Ti surface. Both Lenti-BMP-2 and trehalose showed sustained release over the course of 8 days. Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation. In addition, Lyo-Tre-Lenti-BMP-2 nanotubes effectively inhibited lipopolysaccharide-induced interleukin-1β and tumor necrosis factor-α production. In vivo, the formulation also promoted osseointegration. This study presents a promising new method for surface-modifying biomedical Ti-based implants to simultaneously enhance their osteogenic potential and anti-inflammatory properties, which can better satisfy clinical needs.
为了增强钛植入物与周围骨组织之间的生物相容性和骨整合能力，人们已经做出了许多努力来改变钛植入物的表面形貌和成分。在本文中，通过在海藻糖存在下冻干制备了负载慢病毒介导的骨形态发生蛋白-2（Lenti-BMP-2）的二氧化钛纳米管涂层，以对表面进行功能化处理。我们从以下几个方面对二氧化钛纳米管层进行了表征：表面形态；Lenti-BMP-2和海藻糖的释放；它们在体外诱导成骨、增殖和抗炎的能力；以及在体内的骨整合情况。阳极氧化的二氧化钛纳米管表面呈现出垂直于钛表面的无定形玻璃状基质。Lenti-BMP-2和海藻糖在8天的时间内均表现出持续释放。实时定量聚合酶链反应研究结果表明，用海藻糖构建的冻干Lenti-BMP-2/二氧化钛纳米管（Lyo-Tre-Lenti-BMP-2）显著促进了骨髓基质细胞的成骨分化，但对其增殖没有促进作用。此外，Lyo-Tre-Lenti-BMP-2纳米管有效抑制了脂多糖诱导的白细胞介素-1β和肿瘤坏死因子-α的产生。在体内，该制剂也促进了骨整合。本研究提出了一种有前景的新方法，用于对生物医学钛基植入物进行表面改性，以同时增强其成骨潜力和抗炎特性，从而更好地满足临床需求。
