Katz N R
Institut für Klinische Chemie und Pathobiochemie, Universitätsklinikum Giessen, Germany.
Histochem J. 1989 Sep-Oct;21(9-10):517-29. doi: 10.1007/BF01753352.
A large number of histological, histochemical and biochemical techniques are available for studying liver cell heterogeneity. Structural differences are recognized by morphometric analyses of electron micrographs. The zonal heterogeneity of enzyme activities can be demonstrated by histochemistry and more precisely by ultramicrobiochemical assays in microdissected periportal and perivenous tissue. Immunohistochemistry is useful for quantifying and localizing proteins, especially isoenzymes, without depending on their biological activity. The zonal quantification of specific mRNA can be achieved by in situ hybridization. The different structural and enzymic equipment of periportal and perivenous tissue found by these techniques has led to the concept of metabolic zonation. This hypothesis can be confirmed by determination of metabolic rates in perfused liver after selective zonal damage, in separated periportal and perivenous hepatocytes as well as in periportal and perivenous tissue of perfused liver by non-invasive techniques.
有大量的组织学、组织化学和生物化学技术可用于研究肝细胞异质性。通过对电子显微镜照片进行形态计量分析可识别结构差异。酶活性的区域异质性可通过组织化学来证明，更精确地是通过对显微解剖的门静脉周围和肝静脉周围组织进行超微生化分析来证明。免疫组织化学对于定量和定位蛋白质，特别是同工酶很有用，而无需依赖其生物活性。特定mRNA的区域定量可通过原位杂交来实现。通过这些技术发现的门静脉周围和肝静脉周围组织不同的结构和酶装备导致了代谢分区的概念。通过选择性区域损伤后灌注肝脏的代谢率测定、分离的门静脉周围和肝静脉周围肝细胞以及灌注肝脏的门静脉周围和肝静脉周围组织的非侵入性技术测定，这一假说可以得到证实。
