Paulin-Levasseur M, Scherbarth A, Giese G, Röser K, Bohn W, Traub P
Max-Planck-Institut für Zellbiologie, Ladenburg/Heidelberg, Federal Republic of Germany.
J Cell Sci. 1989 Mar;92 ( Pt 3):361-70. doi: 10.1242/jcs.92.3.361.
Using immunofluorescence and immunoblotting techniques, we have examined the composition of the nuclear lamina in murine plasmacytoma cells, MPC-11, exposed to the phorbol ester TPA as well as in two cell lines devoid of cytoplasmic intermediate filament proteins, the human adrenal cortex carcinoma-derived cells SW-13 and the clone C6-M-D4 derived from the rat glial cell line C6. Our results show that the inhibition of proliferation and the induction of vimentin synthesis observed in TPA-treated MPC-11 populations are not paralleled by changes in the lamin complement of these cells, which contain lamin B but lack lamins A and C. Furthermore, the analysis performed on SW-13 and C6-M-D4 cell lines clearly demonstrates that mammalian somatic cells display considerable variations in lamin expression and indicates that lamin B may be the only lamin species constitutively expressed in mammalian cells.
利用免疫荧光和免疫印迹技术，我们检测了暴露于佛波酯TPA的小鼠浆细胞瘤细胞MPC-11以及两种缺乏细胞质中间丝蛋白的细胞系（源自人肾上腺皮质癌的SW-13细胞和源自大鼠神经胶质细胞系C6的克隆C6-M-D4）中核纤层的组成。我们的结果表明，在TPA处理的MPC-11细胞群体中观察到的增殖抑制和波形蛋白合成诱导与这些细胞的核纤层蛋白组成变化并不平行，这些细胞含有核纤层蛋白B，但缺乏核纤层蛋白A和C。此外，对SW-13和C6-M-D4细胞系进行的分析清楚地表明，哺乳动物体细胞在核纤层蛋白表达上表现出相当大的差异，并表明核纤层蛋白B可能是哺乳动物细胞中组成性表达的唯一核纤层蛋白种类。
