Champagne Antoine, Boutry Marc
Institut des Sciences de la Vie, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.
Institut des Sciences de la Vie, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.
Biochim Biophys Acta. 2016 Aug;1864(8):1039-49. doi: 10.1016/j.bbapap.2016.02.010. Epub 2016 Feb 9.
Among the specialized (secondary) plant metabolites, terpenoids represent the most diverse family and are often involved in the defense against pathogens and herbivores. Terpenoids can be produced both constitutively and in response to the environment. At the front line of this defense strategy are the glandular trichomes, which are organs dedicated primarily to the production of specialized metabolites. Mass spectrometry-based proteomics is a powerful tool, which is very useful to investigate enzymes involved in metabolic pathways, such as the synthesis and secretion of terpenoids in glandular trichomes. Here we review the strategies used to investigate the specific roles of these particular organs from non-model plant species, mainly belonging to the Lamiaceae, Solanaceae, and Cannabaceae families. We discuss how proteomics helps to accurately pinpoint candidate proteins to be functionally characterized, and how technological progresses create opportunities for studying low-abundance proteins, such as the ones related to the synthesis and transport of specialized metabolites. This article is part of a Special Issue entitled: Plant Proteomics--a bridge between fundamental processes and crop production, edited by Dr. Hans-Peter Mock.
在植物次生代谢产物中，萜类化合物是种类最为多样的家族，常参与植物对病原体和食草动物的防御。萜类化合物既可以组成型合成，也可以响应环境刺激而产生。在这种防御策略的前沿是腺毛，它们是主要负责合成特殊代谢产物的器官。基于质谱的蛋白质组学是一种强大的工具，对于研究参与代谢途径的酶非常有用，例如腺毛中萜类化合物的合成和分泌。在此，我们综述了用于研究这些主要来自唇形科、茄科和大麻科非模式植物物种特定器官具体作用的策略。我们讨论了蛋白质组学如何有助于准确确定待进行功能表征的候选蛋白质，以及技术进步如何为研究低丰度蛋白质创造机会，例如与特殊代谢产物合成和运输相关的蛋白质。本文是由汉斯 - 彼得·莫克博士编辑的名为《植物蛋白质组学——基础过程与作物生产之间的桥梁》特刊的一部分。
