Giles Julia, Perrott Matthew, Roe Wendi, Dunowska Magdalena
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Tennent drive, Palmerston North, New Zealand.
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Tennent drive, Palmerston North, New Zealand.
Virology. 2016 Apr;491:20-6. doi: 10.1016/j.virol.2016.01.005. Epub 2016 Feb 10.
The objective of this study was to investigate a role of a recently discovered marsupial nidovirus in the development of a neurological disease, termed wobbly possum disease (WPD), in the Australian brushtail possum (Trichosurus vulpecula). Four possums received 1 mL of a standard inoculum that had been prepared from tissues of WPD-affected possums, 4 possums received 1.8 mL (1 × 10(6) TCID50) of a cell lysate from inoculated cultures, and 4 possums received 1 mL (× 10(7) TCID50) of a purified WPD isolate. All but one possum that received infectious inocula developed neurological disease and histopathological lesions characteristic for WPD. High levels of viral RNA were detected in livers from all possums that received infectious inocula, but not from control possums. Altogether, our data provide strong experimental evidence for the causative involvement of WPD virus in development of a neurological disease in infected animals.
本研究的目的是调查一种最近发现的有袋动物正链RNA病毒在澳大利亚帚尾袋貂（Trichosurus vulpecula）发生的一种被称为袋貂摇摆病（WPD）的神经疾病发展过程中的作用。4只袋貂接种了1毫升由感染WPD的袋貂组织制备的标准接种物，4只袋貂接种了1.8毫升（1×10⁶半数组织培养感染剂量）来自接种培养物的细胞裂解物，4只袋貂接种了1毫升（×10⁷半数组织培养感染剂量）纯化的WPD分离株。除1只接种感染性接种物的袋貂外，其他所有袋貂均出现了WPD特有的神经疾病和组织病理学病变。在所有接种感染性接种物的袋貂肝脏中均检测到高水平的病毒RNA，但在对照袋貂肝脏中未检测到。总之，我们的数据为WPD病毒在受感染动物神经疾病发展过程中的致病作用提供了有力的实验证据。
