Course of Safety Management in Food Supply Chain, Tokyo University of Marine Science and Technology, Tokyo, Japan; Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, Japan.
Course of Safety Management in Food Supply Chain, Tokyo University of Marine Science and Technology, Tokyo, Japan.
Allergol Int. 2016 Jul;65(3):272-9. doi: 10.1016/j.alit.2016.01.002. Epub 2016 Feb 11.
Parvalbumin and collagen have been identified as cross-reactive allergens for fish allergies. Although doctors realize that various fish elicit allergies, the targets of food allergen labeling laws were only mackerels and salmons in Japan and mackerels in South Korea. This study aimed to reveal the causative species for fish allergy via questionnaires and blood tests.
Questionnaire research was conducted in Japan via the internet concerning allergies for fish-allergic patients or their family members. Next, IgE reactivities and cross-reactivities of 26 fish species were analyzed using sera obtained from 16 Japanese patients who were allergic to fish parvalbumin or collagen by enzyme-linked immunosorbent assay (ELISA) and inhibition ELISA.
Questionnaire research revealed that 88% patients cannot eat mackerel and salmon in addition to other fish. In addition, 85% respondents were not satisfied with the current food allergen labeling law. In ELISA analyses, we clarified that pooled serum obtained from patients with fish parvalbumin-specific allergies exhibited IgE reactivity to the extracts of most fish species, and pooled serum obtained from patients with fish collagen-specific allergies displayed IgE reactivity to the extracts of all types of fish. Inhibition ELISA experiments revealed cross-reactivities of parvalbumin or collagen to extracts from all fish tested.
Most patients with fish allergies displayed allergic symptoms following the intake of various fish species. In addition, fish parvalbumin and collagen were causative factors of fish allergy and were highly cross-reactive fish panallergens. Therefore, current laws should be revised in Japan and South Korea.
副肌球蛋白和胶原蛋白已被鉴定为鱼类过敏的交叉反应过敏原。尽管医生意识到各种鱼类都会引发过敏，但日本的食品过敏原标签法仅针对鲐鱼和鲑鱼，韩国则仅针对鲐鱼。本研究旨在通过问卷调查和血液测试来揭示引发鱼类过敏的致敏物种。
通过互联网在日本对鱼类过敏患者或其家庭成员进行了问卷调查研究。然后，通过酶联免疫吸附试验（ELISA）和抑制 ELISA，使用从 16 名对鱼类副肌球蛋白或胶原蛋白过敏的日本患者获得的血清分析了 26 种鱼类的 IgE 反应性和交叉反应性。
问卷调查研究表明，88%的患者除了其他鱼类外，还不能食用鲐鱼和鲑鱼。此外，85%的受访者对现行的食品过敏原标签法不满意。在 ELISA 分析中，我们阐明了从鱼类副肌球蛋白特异性过敏患者的混合血清中获得的血清对大多数鱼类的提取物表现出 IgE 反应性，而从鱼类胶原蛋白特异性过敏患者的混合血清中获得的血清对所有类型的鱼类提取物均表现出 IgE 反应性。抑制 ELISA 实验表明副肌球蛋白或胶原蛋白与所有测试鱼类的提取物具有交叉反应性。
大多数鱼类过敏患者在摄入各种鱼类后会出现过敏症状。此外，鱼类副肌球蛋白和胶原蛋白是鱼类过敏的致病因素，并且是高度交叉反应的鱼类泛过敏原。因此，日本和韩国的现行法律应予以修订。
