基于适配体的“即时检测”。

Aptamer-based 'point-of-care testing'.

机构信息

Institute of Nano Electronic Engineering (INEE), Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia; School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis, Malaysia.

Institute of Nano Electronic Engineering (INEE), Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia.

出版信息

Biotechnol Adv. 2016 May-Jun;34(3):198-208. doi: 10.1016/j.biotechadv.2016.02.003. Epub 2016 Feb 10.

DOI:10.1016/j.biotechadv.2016.02.003

PMID:26876017

Abstract

Aptamers are single-stranded oligonucleotides that can be artificially generated by a method called Systematic evolution of ligands by exponential enrichment (SELEX). The generated aptamers have been assessed for high-performance sensing applications due to their appealing characteristics. With either aptamers alone or complementing with antibodies, several high sensitive and portable sensors have been demonstrated for use in 'point-of-care testing'. Due to their high suitability and flexibility, aptamers are conjugated with nanostructures and utilized in field applications. Moreover, aptamers are more amenable to chemical modifications, making them capable of utilization with most developed sensors. In this overview, we discuss novel, portable, and aptamer-based sensing strategies that are suitable for 'point-of-care testing'.

摘要

适体是单链寡核苷酸，可以通过一种称为指数富集配体系统进化（SELEX）的方法人工生成。由于具有吸引人的特性，所生成的适体已被评估用于高性能传感应用。通过单独使用适体或与抗体互补，已经展示了几种高灵敏度和便携式传感器，用于“即时检测”。由于其高度适用性和灵活性，适体与纳米结构结合并应用于现场应用。此外，适体更适合化学修饰，使其能够与大多数开发的传感器一起使用。在这篇综述中，我们讨论了适合“即时检测”的新型、便携式和基于适体的传感策略。