Li Zhen, Hu Bo, Zhou Rong, Zhang Xiaojuan, Wang Ruizhe, Gao Yun, Sun Mingjuan, Jiao Binghua, Wang Lianghua
Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Navy Medical University Shanghai 200433 P. R. China
Department of Marine Biomedicine and Polar Medicine, Naval Special Medical Center Shanghai 200433 P. R. China.
RSC Adv. 2020 Feb 26;10(14):8181-8189. doi: 10.1039/c9ra10600f. eCollection 2020 Feb 24.
Diarrhetic shellfish toxins (DSTs) are marine toxins distributed widely in the world, which pose a major threat to the health of mankind. Dinophysistoxin-1 (DTX-1) has the most potent toxicity in DSTs. However, the current detection methods have ethical problems and technical defects. Further research is needed, to develop a more suitable alternative to the supervision system. In this work, we successfully obtained an aptamer with high affinity and specificity bound to DTX-1 for the first time. After optimization, a core sequence of the aptamer with a higher of 64 nM was obtained, while the binding mode of the core sequence and DTX-1 was explored. Based on this aptamer, we developed a biolayer interferometry (BLI) biosensor platform for DTX-1 detection. The aptasensor exhibited a broad detection range from 40 to 600 nM DTX-1 (linear range from 80 to 200 nM), and the low detection limit was 614 pM. Morever, the aptasensor showed good reproducibility and stability, which indicated that this novel aptasensor had broad development prospects for the sensitive and rapid detection of DTX-1.
腹泻性贝类毒素（DSTs）是广泛分布于全球的海洋毒素，对人类健康构成重大威胁。鳍藻毒素-1（DTX-1）在腹泻性贝类毒素中具有最强的毒性。然而，目前的检测方法存在伦理问题和技术缺陷。需要进一步研究，以开发更合适的替代监测系统。在这项工作中，我们首次成功获得了一种与DTX-1具有高亲和力和特异性的适体。经过优化，获得了适体的核心序列，其解离常数为64 nM，同时探索了核心序列与DTX-1的结合模式。基于这种适体，我们开发了一种用于检测DTX-1的生物层干涉术（BLI）生物传感器平台。该适体传感器对DTX-1的检测范围为40至600 nM（线性范围为80至200 nM），检测下限为614 pM。此外，该适体传感器具有良好的重现性和稳定性，这表明这种新型适体传感器在DTX-1的灵敏快速检测方面具有广阔的发展前景。
