Esposito V, Pepe A, Filosa R, Mayol L, Virgilio A, Galeone A
Department of Pharmacy, University of Naples "Federico II", Via D. Montesano, 49, 80131 Naples, Italy.
Department of Science, University of Basilicata, Viale dell'Ateneo Lucano 10, I-85100, Potenza, Italy.
Org Biomol Chem. 2016 Mar 14;14(10):2938-43. doi: 10.1039/c5ob02358k.
G-quadruplex structures formed by oligodeoxyribonucleotides TGGU(NH2)GGT (AM, U(NH2) = 5-amino-2'-deoxyuridine), TGGU(Br)GGT (BR, U(Br) = 5-bromo-2'-deoxyuridine) and TGGTGGT (TH) have been investigated through circular dichroism, nuclear magnetic resonance, gel electrophoresis and molecular modeling techniques. Collected data indicate that all 7-mer oligonucleotides form tetramolecular parallel G-quadruplex structures with all residues adopting anti glycosidic bonds. In the case of AM, data suggest the occurrence of a novel U(NH2)-tetrad characterized by eight hydrogen bonds that stabilizes the G-quadruplex structure more efficiently than U(Br)- and T-tetrads.
通过圆二色性、核磁共振、凝胶电泳和分子建模技术，对由寡脱氧核糖核苷酸TGGU(NH2)GGT（AM，U(NH2)=5-氨基-2'-脱氧尿苷）、TGGU(Br)GGT（BR，U(Br)=5-溴-2'-脱氧尿苷）和TGGTGGT（TH）形成的G-四链体结构进行了研究。收集的数据表明，所有7聚体寡核苷酸均形成四分子平行G-四链体结构，所有残基均采用反式糖苷键。对于AM，数据表明存在一种新型的U(NH2)-四联体，其特征是有八个氢键，比U(Br)-和T-四联体更有效地稳定G-四链体结构。
