Wesołowski Piotr, Zawada Katarzyna, Wojtowicz Andrzej, Strużycka Izabela, Kamiński Tomasz
Department of Oral Surgery, Medical University of Warsaw, Warsaw, Poland.
Department of Physical Chemistry, Medical University of Warsaw, Warsaw, Poland.
J Oral Pathol Med. 2016 Nov;45(10):753-757. doi: 10.1111/jop.12433. Epub 2016 Feb 14.
Oxidative DNA damage leads to abnormalities in endogenous cellular processes and constitutes a direct link between free radicals, antioxidants and squamous cell carcinoma (SCC). Oxygen radical absorbance capacity (ORAC) method is used to measure antioxidant capacity versus oxygen free radicals. There is no report on the application of ORAC to evaluate salivary antioxidant capacity in patients with head and neck SCC. This study investigated the relation between total antioxidant capacity of saliva, measured with ORAC method, and the incidence, location, size and histopathological grading of SCC.
Salivary antioxidant capacity was assessed in 30 male and female patients with histopathologically confirmed oral and laryngeal SCC. Histopathological grading of SCC was based on WHO classification.
Mean ORAC in patients with SCC was higher than the mean value in the control group. Statistically significant difference was observed in the study group versus the control group. No significant differences were observed for ORAC values in relation to histopathological grading, tumour size and spread to lymph nodes.
The obtained results can advocate local compensatory mechanism in saliva in response to increased oxidative stress associated with primary tumour. ORAC can become an additional factor in head and neck SCC prognosis, but further investigation is required.
氧化性DNA损伤会导致内源性细胞过程异常,并构成自由基、抗氧化剂与鳞状细胞癌(SCC)之间的直接联系。氧自由基吸收能力(ORAC)方法用于测量抗氧化剂对抗氧自由基的能力。目前尚无关于应用ORAC评估头颈部SCC患者唾液抗氧化能力的报道。本研究调查了用ORAC方法测量的唾液总抗氧化能力与SCC的发病率、位置、大小及组织病理学分级之间的关系。
对30例经组织病理学确诊为口腔和喉SCC的男性和女性患者的唾液抗氧化能力进行评估。SCC的组织病理学分级基于世界卫生组织的分类。
SCC患者的平均ORAC高于对照组的平均值。研究组与对照组之间观察到统计学上的显著差异。未观察到ORAC值在组织病理学分级、肿瘤大小及淋巴结转移方面的显著差异。
所获结果表明,唾液中存在局部代偿机制以应对与原发性肿瘤相关的氧化应激增加。ORAC可能成为头颈部SCC预后的一个附加因素,但仍需进一步研究。