Department of Clinical Pathomorphology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz, Poland.
Department of Otolaryngology and Clinical Oncology Chair and Clinic of Otolaryngology and Department of Pathophysiology of Hearing and Balance System Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz, Poland.
Adv Med Sci. 2014 Sep;59(2):206-12. doi: 10.1016/j.advms.2014.03.005. Epub 2014 Jun 9.
Laryngeal squamous cell carcinoma (LSCC) is an interesting diagnostic and therapeutic issue. The diagnostic delay is mainly a consequence of the lack of evident symptoms in the early stage of the disease. The purpose of current studies was the evaluation of the expression of p27(kip1) in primary and metastatic LSCC in correlation with patients' clinicopathological data.
MATERIAL/METHODS: The indirect immunohistochemical studies were performed on the series of 60 sections (primary tumor: 20 cases of N(0) and 20 cases of N(+), and nodal meta: 20 cases), using primary antibody against p27(kip1) [clone 1B4]. The expression of analyzed protein was performed using automated morphometric methods.
The p27(kip1) nuclear expression was found in 100% (40/40) cases of primary tumor, and in 85% (17/20) cases of SCC meta at lymph nodes. In primary LSCC N(0) the expression of p27(kip1) was significantly higher compared to N(+) cases (p=0.036672). However, the p27(kip1) expression in SCC metastases was higher compared to the primary SCC. Moreover, the analyses based on the classification trees revealed the cutoff p27(kip1) expression in primary LSCC (IRS ≤ 76) which was characteristic for N(+) patients. Consequently, our analysis revealed that high expression of p27(kip1) (IRS>76) was characteristic for N(0) patients.
Our results suggest that p27(kip1) might be useful prognostic factor of metastatic potential in laryngeal squamous cell carcinoma.
喉鳞状细胞癌（LSCC）是一个有趣的诊断和治疗问题。诊断延迟主要是由于疾病早期缺乏明显症状所致。目前研究的目的是评估原发性和转移性 LSCC 中 p27(kip1)的表达与患者临床病理数据的相关性。
材料/方法：对 60 个切片系列（原发性肿瘤：20 例 N(0)和 20 例 N(+)，以及淋巴结转移：20 例）进行间接免疫组织化学研究，使用针对 p27(kip1) [克隆 1B4]的抗体。采用自动形态计量学方法对分析蛋白的表达进行检测。
在 40/40 例原发性肿瘤和 17/20 例淋巴结 SCC 转移病例中发现了 p27(kip1)核表达。在原发性 LSCC N(0)中，p27(kip1)的表达明显高于 N(+)病例（p=0.036672）。然而，SCC 转移中的 p27(kip1)表达高于原发性 SCC。此外，基于分类树的分析揭示了原发性 LSCC 中 p27(kip1)表达的截断值（IRS≤76），这是 N(+)患者的特征。因此，我们的分析表明，p27(kip1)高表达（IRS>76）是 N(0)患者的特征。
我们的结果表明，p27(kip1)可能是喉鳞状细胞癌转移潜能的有用预后因素。
