Hao Limin, Sheng Zhicun, Lu Jike, Tao Ruyu, Jia Shiru
The Quartermaster Equipment Institute of General Logistics Department of People's Liberation Army, Beijing 100010, China; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457 China.
College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457 China.
Carbohydr Polym. 2016 May 5;141:54-9. doi: 10.1016/j.carbpol.2015.11.048. Epub 2015 Nov 19.
Fomitopsis pinicola (F. pinicola) is a kind of medicinal fungi, and few studies has been carried out on F. pinicola polysaccharides from liquid submerged cultivation. The characterization and antioxidant activities of extracellular polysaccharide (EPS) and intracellular polysaccharide (IPS) isolated from F. pinicola were investigated. The results showed that the molecular weight of EPS was 2.30×10(4)Da, and EPS was composed of mannose, rhamnose, xylose and galactose with the molar ratio of 0.1:1.0:0.3:0.5. The molecular weight of IPS was 4.07×10(5)Da, and the monosaccharide compositions included glucose, mannose, rhamnose, xylose and galactose with the molar ratio of 1.0:0.9:0.9:0.8:1.1. Antioxidant activities of both EPS and IPS including in vitro scavenging activities on 1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals, cellular protective effects on yeast cells from ultraviolet (UV) radiation and H2O2 oxidative damage were tested. Both EPS and IPS showed antioxidant activities in a dose dependent manner, and IPS had higher antioxidant activity than EPS. So EPS and IPS could be potential novel antioxidants for functional food.
松生拟层孔菌是一种药用真菌，关于液体深层培养的松生拟层孔菌多糖的研究较少。本文对松生拟层孔菌胞外多糖（EPS）和胞内多糖（IPS）的特性及抗氧化活性进行了研究。结果表明，EPS的分子量为2.30×10⁴Da，由甘露糖、鼠李糖、木糖和半乳糖组成，摩尔比为0.1:1.0:0.3:0.5。IPS的分子量为4.07×10⁵Da，单糖组成包括葡萄糖、甘露糖、鼠李糖、木糖和半乳糖，摩尔比为1.0:0.9:0.9:0.8:1.1。测试了EPS和IPS的抗氧化活性，包括对1,1-二苯基-2-三硝基苯肼（DPPH）和羟基自由基的体外清除活性，以及对酵母细胞免受紫外线（UV）辐射和H₂O₂氧化损伤的细胞保护作用。EPS和IPS均呈现出剂量依赖性的抗氧化活性，且IPS的抗氧化活性高于EPS。因此，EPS和IPS可能是功能性食品潜在的新型抗氧化剂。
