Fainstein E, Einat M, Gokkel E, Marcelle C, Croce C M, Gale R P, Canaani E
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Oncogene. 1989 Dec;4(12):1477-81.
The complete nucleotide sequence of human abl RNA containing exon Ia was determined. It spans 5598 nucleotides and codes for a protein of 1130 amino acids. The 3' untranslated region contains two short open reading frames and multiple ATTT(A) motifs characteristic of short lived mRNAs. Computer analysis of the abl protein predicts four domains distinct with regard to surface probability and chain flexibility. Nucleotide analysis of the abl segment within a bcr-abl cDNA cloned from the K562 cell line indicated no further alterations within the coding region. A bcr-abl construct containing this segment transformed, together with c-myc, RAT-1 cells and produced a highly active tyrosine kinase.
测定了包含外显子Ia的人abl RNA的完整核苷酸序列。它全长5598个核苷酸，编码一个由1130个氨基酸组成的蛋白质。3'非翻译区包含两个短开放阅读框以及多个短命mRNA特有的ATTT(A)基序。对abl蛋白的计算机分析预测出四个在表面概率和链柔韧性方面不同的结构域。对从K562细胞系克隆的bcr-abl cDNA内的abl片段进行核苷酸分析表明，编码区内没有进一步的改变。包含该片段的bcr-abl构建体与c-myc一起转化RAT-1细胞，并产生了一种高活性酪氨酸激酶。
