长期高脂饮食摄入对雌性大鼠外周胰岛素敏感性、血压和肾功能的影响。
Effect of long-term high-fat diet intake on peripheral insulin sensibility, blood pressure, and renal function in female rats.
作者信息
Roza Noemi A V, Possignolo Luiz F, Palanch Adrianne C, Gontijo José A R
机构信息
Laboratório de Metabolismo Hidrossalino, Núcleo de Medicina e Cirurgia Experimental, Departamento de Clínica Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, Brazil.
Laboratório de Metabolismo Hidrossalino, Núcleo de Medicina e Cirurgia Experimental, Departamento de Clínica Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, Brazil;
出版信息
Food Nutr Res. 2016 Feb 12;60:28536. doi: 10.3402/fnr.v60.28536. eCollection 2016.
BACKGROUND
This study determines whether 8-week high-fat diet (HFD) consumption alters insulin sensitivity, kidney function, and blood pressure (BP) in female rats when compared with standard rodent diet (ND) intake in gender- and age-matched rats.
METHODS
The present study investigates, in female Wistar HanUnib rats, the effect of long-term high-fat fed group (HFD) compared with standard chow on BP by an indirect tail-cuff method using an electrosphygmomanometer, insulin and glucose function, and kidney function by creatinine and lithium clearances.
RESULTS
The current study shows glucose tolerance impairment, as demonstrated by increased fasting blood glucose (ND: 78±2.8 vs. HFD: 87±3.8 mg/dL) associated with reduced insulin secretion (ND: 0.58±0.07 vs. HFD: 0.40±0.03 ng/mL) in 8-week female HFD-treated rats. The incremental area under the curve (AUC, ND: 1,4558.0±536.0 vs. HFD: 1,6507.8±661.9), homeostasis model assessment of insulin resistance (HOMA-IR) index, and the first-order rate constant for the disappearance of glucose (Kitt) were significantly enhanced in 8-week HFD-treated rats compared with age-matched ND group (respectively, P=0.03, P=0.002, and P<0.0001). The current study also shows a significantly higher systolic BP measured in 5 and 8 weeks posttreatment in HFD (5-week HFD-treated: 155.25±10.54 mmHg and 8-week HFD-treated: 165±5.8 mmHg) (P=0.0001), when compared to BP values in 5-week ND, 137±4.24 mmHg and 8-week ND, 131.75±5.8 mmHg age-matched group. Otherwise, the glomerular filtration rate and renal sodium handling evaluated by FENa, FEPNa and FEPPNa, were unchanged in both groups.
CONCLUSION
We may conclude that 8-week female HFD-fed rats compared with ND group stimulate harmful effects, such as BP rise and peripheral glucose intolerance. The increased BP occurs through insulin resistance and supposedly decreased vasodilatation response without any change on renal function.
背景
本研究旨在确定与性别和年龄匹配的大鼠摄入标准啮齿动物饮食（ND）相比，雌性大鼠食用8周高脂饮食（HFD）是否会改变胰岛素敏感性、肾功能和血压（BP）。
方法
本研究在雌性Wistar HanUnib大鼠中，通过使用电子血压计的间接尾袖法，研究长期高脂喂养组（HFD）与标准饲料相比对血压的影响，以及通过肌酐清除率和锂清除率研究胰岛素和葡萄糖功能及肾功能。
结果
当前研究显示，8周龄雌性HFD处理大鼠出现糖耐量受损，表现为空腹血糖升高（ND：78±2.8 vs. HFD：87±3.8 mg/dL），同时胰岛素分泌减少（ND：0.58±0.07 vs. HFD：0.40±0.03 ng/mL）。与年龄匹配的ND组相比，8周龄HFD处理大鼠的曲线下增量面积（AUC，ND：14558.0±536.0 vs. HFD：16507.8±661.9）、胰岛素抵抗稳态模型评估（HOMA-IR）指数以及葡萄糖消失的一级速率常数（Kitt）均显著升高（分别为P = 0.03、P = 0.002和P < 0.0001）。当前研究还显示，与5周龄ND组（137±4.24 mmHg）和8周龄ND组（131.75±5.8 mmHg）的年龄匹配组血压值相比，HFD组在治疗后5周和8周测量的收缩压显著更高（5周龄HFD处理：155.25±10.54 mmHg和8周龄HFD处理：165±5.8 mmHg）（P = 0.0001）。此外，两组通过FENa、FEPNa和FEPPNa评估的肾小球滤过率和肾钠处理均未改变。
结论
我们可以得出结论，与ND组相比，8周龄雌性HFD喂养大鼠会产生有害影响，如血压升高和外周糖耐量异常。血压升高是通过胰岛素抵抗发生的，推测血管舒张反应降低，而肾功能没有任何变化。
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