Ukabam C U
Department of Physiology, University of Bristol Medical School.
Ric Clin Lab. 1989 Jul-Sep;19(3):273-80. doi: 10.1007/BF02871817.
The hypoglossal nucleus in 129 REJ normal mouse strains was investigated using two neuroanatomical markers, namely the cobalt chloride (CoCl2) and the horseradish peroxidase (HRP) techniques. CoCl2 was introduced through the cut end of the hypoglossal nerve. In one set of experiments HRP was injected into the hypoglossal nerve, while in the other it was injected into the tongue musculature. Results show that with these techniques the hypoglossal neurons are conspicuously stained and can be easily located among series of brainstem sections. The mean number +/- SD of neurons in the hypoglossal nucleus was 1,417 +/- 37, 846 +/- 28 and 1,272 +/- 42 using CoCl2 and HRP injected into the tongue musculature or the hypoglossal nerve, respectively. The estimated length of the nucleus was 0.92 mm with the CoCl2 technique.
使用两种神经解剖学标记物，即氯化钴（CoCl2）和辣根过氧化物酶（HRP）技术，对129个REJ正常小鼠品系的舌下神经核进行了研究。CoCl2通过舌下神经的切断端注入。在一组实验中，将HRP注入舌下神经，而在另一组实验中，将其注入舌肌组织。结果表明，使用这些技术，舌下神经元被显著染色，并且在一系列脑干切片中很容易定位。分别使用注入舌肌组织或舌下神经的CoCl2和HRP，舌下神经核中神经元的平均数量±标准差为1417±37、846±28和1272±42。使用CoCl2技术时，该核的估计长度为0.92毫米。
