Guo Mingxiao, Lu Chunlei, Gao Ying, Zhang Haifeng, Chen Dongfeng, Li Yousheng
Department of Laparoscopic Surgery Center, Linyi People's Hospital, Shandong University, Linyi 276000, China.
Department of Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
Gastroenterol Res Pract. 2016;2016:3925751. doi: 10.1155/2016/3925751. Epub 2016 Jan 12.
Background and Objectives. The intestinal mucosa is extremely sensitive to ischemia. Better intestinal preservation is the first step to improve the results of intestinal transplantation. The aim of the study is to investigate the effect of cold Lifor solution on preservation of swine small bowel. Methods. Swine ileum segments (200 cm) were allotransplanted heterotopically after 9-hour cold storage with UW solution (group 1, n = 6), with Lifor solution (group 2, n = 6), or without storage (group 3, n = 6), respectively. After cold storage, mucosal adenosine triphosphate (ATP) concentrations and histopathologic analysis after preservation were performed. At day 7 after the transplantation, intestinal absorptive function was also observed. Results. After 9 h cold preservation, pathological changes, the content of ATP in the intestinal mucosa, and the intestinal absorptive function after transplantation in group 2 were similar to those of group 1. Conclusion. The effect of cold storage of swine small bowel with Lifor solution is similar to that of UW solution. It may provide additional rationale for further exploration of Lifor as an alternative preservation solution in small bowel transplantation.
背景与目的。肠黏膜对缺血极为敏感。更好地保存肠道是改善肠道移植效果的首要步骤。本研究旨在探讨冷的利弗溶液对猪小肠保存的影响。方法。将猪回肠段（200厘米）分别用UW溶液冷保存9小时后进行异位同种异体移植（第1组，n = 6），用利弗溶液冷保存9小时后进行异位同种异体移植（第2组，n = 6），或不进行冷保存直接移植（第3组，n = 6）。冷保存后，检测黏膜三磷酸腺苷（ATP）浓度并进行保存后的组织病理学分析。在移植后第7天，还观察肠道吸收功能。结果。冷保存9小时后，第2组的病理变化、肠黏膜中ATP含量以及移植后的肠道吸收功能与第1组相似。结论。用利弗溶液冷保存猪小肠的效果与UW溶液相似。这可能为进一步探索利弗作为小肠移植替代保存液提供额外的理论依据。
