Saadah Omar I, Al-Harthi Sameer E, Al-Mughales Jamil A, Bin-Taleb Yagoub Y, Baeshen Raed S
Division of Pediatric Gastroenterology / Department of Pediatrics, Faculty of Medicine, King Abdulaziz UniversityJeddah, Saudi Arabia; Inflammatory Bowel Disease Research Group, King Abdulaziz UniversityJeddah, Saudi Arabia.
Department of Pharmacology, Faculty of Medicine, King Abdulaziz UniversityJeddah, Saudi Arabia; Inflammatory Bowel Disease Research Group, King Abdulaziz UniversityJeddah, Saudi Arabia.
Int J Clin Exp Pathol. 2015 Dec 1;8(12):16000-6. eCollection 2015.
Interleukin-33 (IL-33) is a cytokine that belongs to the interleukin-1 family and has been shown to be associated with mucosal inflammation. The aim of this study was to determine the serum level of IL-33 in children with ulcerative colitis (UC) and Crohn's disease (CD) and to correlate the level with the disease progression. In this cross sectional prospective study, we enrolled 50 children with IBD from KAUH, Jeddah, Saudi Arabia and 34 healthy control subjects between June 2012 and December 2012. Serum IL-33 was assessed by ELISA and CRP by immunonephelometric assay. Results from our study showed 32 CD and 18 UC patients included. The median age was 13.5 years for CD patients, 11.9 years for UC patients and 11.2 years for controls. Females constituted 53%, 66.7% and 59% of CD, UC and control subjects respectively. The median serum IL-33 in UC patients of 55.5 pg/mL was significantly higher than the median IL-33 level of 41 pg/mL in the healthy control (P=0.04) but no significant difference was found between the median IL-33 level in the sera of CD and the control group (P=0.7). A higher median IL-33 level was also found in active disease (P=0.03). In our cohort, the serum level of IL-33 was positively correlated with hs-CRP (r=0.48, P < 0.001). To conclude, our results support that serum IL-33 level is increased in children with UC as compared with control. Serum level is correlated with the disease activity; therefore it could be used as a potential biomarker for monitoring the severity of the disease in children with UC.
白细胞介素-33（IL-33）是一种属于白细胞介素-1家族的细胞因子，已被证明与黏膜炎症有关。本研究的目的是测定溃疡性结肠炎（UC）和克罗恩病（CD）患儿血清中IL-33的水平，并将该水平与疾病进展相关联。在这项横断面前瞻性研究中，我们于2012年6月至2012年12月期间从沙特阿拉伯吉达的KAUH招募了50例IBD患儿和34名健康对照者。通过ELISA法评估血清IL-33，通过免疫比浊法测定CRP。我们的研究结果显示纳入了32例CD患者和18例UC患者。CD患者的中位年龄为13.5岁，UC患者为11.9岁，对照组为11.2岁。女性分别占CD、UC和对照组的53%、66.7%和59%。UC患者血清IL-33的中位数为55.5 pg/mL，显著高于健康对照组的中位数41 pg/mL（P = 0.04），但CD患者血清IL-33的中位数水平与对照组之间未发现显著差异（P = 0.7）。在活动期疾病中也发现较高的IL-33中位数水平（P = 0.03）。在我们的队列中，血清IL-33水平与hs-CRP呈正相关（r = 0.48，P < 0.001）。总之，我们的结果支持与对照组相比，UC患儿血清IL-33水平升高。血清水平与疾病活动相关；因此它可作为监测UC患儿疾病严重程度的潜在生物标志物。
