胃癌及癌前病变的微卫星不稳定性
Microsatellite instability of gastric cancer and precancerous lesions.
作者信息
Li Bing, Liu Hong-Yi, Guo Shao-Hua, Sun Peng, Gong Fang-Ming, Jia Bao-Qing
机构信息
Department of Surgical Oncology, General Hospital of The People's Liberation Army No. 28, Fuxing Road, Beijing 100853, P.R. China.
出版信息
Int J Clin Exp Med. 2015 Nov 15;8(11):21138-44. eCollection 2015.
OBJECTIVE
To investigate whether microsatellite instability (MSI) of gastric cancer and precancerous lesions were existed and its effect.
METHODS
Laser microdissection was used. Gastric, intestinal metaplasia, dysplasia and normal mucosa were collected respectively. Five microsatellite loci were selected and MSI was detected by denaturing high-performance liquid chromatography.
RESULTS
In the five microsatellite loci REF-positive phenotype, intestinal metaplasia MSI was 20.7%. Dysplasia MSI was 22.4%. Gastric MSI was 47.9%, and there was no MSI in normal gastric mucosa.
CONCLUSION
MSI gradually increased from precancerous lesions to gastric cancer. The early detection of MSI may be a potential early warning indicator for early diagnosis of gastric cancer.
目的
探讨胃癌及癌前病变中微卫星不稳定性（MSI）的存在情况及其影响。
方法
采用激光显微切割技术，分别收集胃组织、肠化生、异型增生及正常黏膜组织。选取5个微卫星位点，通过变性高效液相色谱法检测MSI。
结果
在5个微卫星位点REF阳性表型中，肠化生的MSI为20.7%，异型增生的MSI为22.4%，胃癌的MSI为47.9%，正常胃黏膜无MSI。
结论
MSI从癌前病变到胃癌呈逐渐升高趋势。MSI的早期检测可能是胃癌早期诊断的潜在预警指标。
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