Department of Gastroenterology, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, China.
Department of Pathology, Renmin Hospital, Wuhan University, Wuhan, 430060, Hubei, China.
Sci Rep. 2023 Aug 1;13(1):12495. doi: 10.1038/s41598-023-39255-7.
Gastric cancer (GC) is a common digestive tract malignancy with the sixth global incidence and third cancer-related deaths, respectively. Microsatellite instability (MSI), accounting for one of the molecular subtypes of GC, plays an important role in GC and is affected by a sophisticated network of gene interactions. In this study, we aimed to explore the expression pattern and clinical performance of MSI related gene in GC patients. Weighted gene co-expression network analysis (WGCNA) was exploited to single out the vital module and core genes in TCGA database. We applied the protein-protein interaction (PPI) and survival analysis to propose and confirm RNF150 as the hub gene in GC. Finally, we utilized immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR) to explore the expression pattern of RNF150 in GC patients. With the highest weight correlation and standard correlation, RNF150 was selected as the hub gene for following validation. In validation, data obtained from the test sets showed a lower expression of RNF150 in MSI GC compared to microsatellite stability (MSS) GC. Moreover, survival analysis shows that MSI GC patients with a lower RNF150 expression level displayed the longer OS time. Compared to the expression in normal gastric tissues, the protein level of RNF150 was virtually up-regulated in ten cases of GC tissues. Furthermore, RNF150 protein level was decreased in MSI GC samples compared to MSS GC samples. When validated the mRNA expression with RT-PCR in fresh GC tissues, we also found the similar trend. RNF150 was identified as a novel MSI-related gene in GC. It is expected to be an auspicious prognostic biomarker for GC patients.
胃癌（GC）是一种常见的消化道恶性肿瘤，其全球发病率排名第六，癌症相关死亡率排名第三。微卫星不稳定（MSI）是 GC 的分子亚型之一，在 GC 中发挥重要作用，并受到基因相互作用的复杂网络的影响。在本研究中，我们旨在探讨 MSI 相关基因在 GC 患者中的表达模式和临床表现。利用加权基因共表达网络分析（WGCNA）从 TCGA 数据库中筛选出关键模块和核心基因。我们应用蛋白质-蛋白质相互作用（PPI）和生存分析，提出并证实 RNF150 是 GC 的枢纽基因。最后，我们利用免疫组织化学（IHC）和逆转录-聚合酶链反应（RT-PCR）来探讨 RNF150 在 GC 患者中的表达模式。RNF150 被选为后续验证的枢纽基因，其具有最高的权重相关性和标准相关性。在验证中，从测试集中获得的数据显示，MSI GC 中 RNF150 的表达水平低于微卫星稳定性（MSS）GC。此外，生存分析表明，RNF150 表达水平较低的 MSI GC 患者具有更长的 OS 时间。与正常胃组织相比，RNF150 在 10 例 GC 组织中的蛋白水平几乎上调。此外，与 MSS GC 样本相比，MSI GC 样本中 RNF150 蛋白水平降低。当用 RT-PCR 在新鲜 GC 组织中验证 mRNA 表达时，我们也发现了类似的趋势。RNF150 被鉴定为 GC 中的一种新型 MSI 相关基因。它有望成为 GC 患者有前途的预后生物标志物。
