神经科学家的设计型受体特异性激活蛋白
DREADDs for Neuroscientists.
作者信息
Roth Bryan L
机构信息
Department of Pharmacology, University of North Carolina Chapel Hill Medical School, Chapel Hill, NC 27514, USA.
出版信息
Neuron. 2016 Feb 17;89(4):683-94. doi: 10.1016/j.neuron.2016.01.040.
Abstract
To understand brain function, it is essential that we discover how cellular signaling specifies normal and pathological brain function. In this regard, chemogenetic technologies represent valuable platforms for manipulating neuronal and non-neuronal signal transduction in a cell-type-specific fashion in freely moving animals. Designer Receptors Exclusively Activated by Designer Drugs (DREADD)-based chemogenetic tools are now commonly used by neuroscientists to identify the circuitry and cellular signals that specify behavior, perceptions, emotions, innate drives, and motor functions in species ranging from flies to nonhuman primates. Here I provide a primer on DREADDs highlighting key technical and conceptual considerations and identify challenges for chemogenetics going forward.
摘要
为了理解大脑功能，我们必须发现细胞信号传导如何决定正常和病理性大脑功能。在这方面，化学遗传学技术是宝贵的平台，可在自由活动的动物中以细胞类型特异性方式操纵神经元和非神经元信号转导。基于设计药物特异性激活的设计受体（DREADD）的化学遗传学工具，目前被神经科学家广泛用于识别从果蝇到非人类灵长类动物等物种中决定行为、感知、情绪、先天驱力和运动功能的神经回路和细胞信号。在此，我提供了一份关于DREADD的入门介绍，强调关键的技术和概念考量，并指出化学遗传学未来面临的挑战。
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