Department of Virology, HIV and Hepatitis Research Unit, Sefako Makgatho Health Sciences University and National Health Laboratory Service, Pretoria, South Africa.
South African Medical Research Council, Pretoria, Gauteng, South Africa.
J Med Virol. 2016 Sep;88(9):1560-6. doi: 10.1002/jmv.24502. Epub 2016 Mar 8.
Hepatitis B virus (HBV) and human immunodeficiency virus (HIV) infections are highly endemic in South Africa. Data on the complete genome sequences of HBV in HIV-positive patients in South Africa are scanty. This study characterized the complete HBV genome isolated from both HIV-positive and negative patients at the Dr George Mukhari Academic Hospital (DGMAH), Pretoria. Serum samples from nine (five HIV-positive and four HIV-negative) patients attending the DGMAH from 2007 to 2011 were serologically tested, amplified, and sequenced for complete genome. Phylogenetic tree was constructed using MEGA6.0. Mutations were analyzed by comparing the sequences with genotype-matched GenBank references. Eight patients were HBsAg positive, with only one from the HIV positive group being negative. Phylogenetic analysis of the complete genome sequences classified them into five genotypes; A1 (n = 4), A2 (n = 1), C1 (n = 2), D1 (n = 1), and D3 (n = 1). Deletions up to 35 nucleotides in length were identified in this study. No drug resistance mutations were identified in the P ORF, while the L217R mutation was identified in one subgenotype A2 sequence. The double (A1762T/G1764A) and triple (T1753C/A1762T/G1764A) mutations in the Basal core promoter were identified in four and two sequences, respectively. In the core region, mutation G1888A was identified in four of the subgenotype A1 sequences. In conclusion, this study has added to the limited South African data on HBV genotypes and mutations in HBV/HIV co-infected and HBV mono-infected patients, based on complete HBV genome analysis. Subgenotype A1 was predominant, and no drug-resistant mutants were detected in the study. J. Med. Virol. 88:1560-1566, 2016. © 2016 Wiley Periodicals, Inc.
乙型肝炎病毒（HBV）和人类免疫缺陷病毒（HIV）感染在南非高度流行。南非 HIV 阳性患者中 HBV 完整基因组序列的数据很少。本研究对来自比勒陀利亚 Dr George Mukhari 学术医院（DGMAH）的 HIV 阳性和阴性患者中分离的 HBV 完整基因组进行了特征描述。从 2007 年至 2011 年在 DGMAH 就诊的 9 名（5 名 HIV 阳性和 4 名 HIV 阴性）患者的血清样本进行了血清学检测、扩增和完整基因组测序。使用 MEGA6.0 构建系统发生树。通过将序列与基因型匹配的 GenBank 参考序列进行比较，分析突变。8 例患者 HBsAg 阳性，其中仅 1 例来自 HIV 阳性组为阴性。完整基因组序列的系统发生分析将其分为 5 个基因型；A1（n = 4）、A2（n = 1）、C1（n = 2）、D1（n = 1）和 D3（n = 1）。在本研究中鉴定出长达 35 个核苷酸的缺失。在 P 基因 ORF 中未发现耐药突变，而在一个亚基因型 A2 序列中发现了 L217R 突变。在四个和两个序列中分别鉴定出基本核心启动子中的双（A1762T/G1764A）和三（T1753C/A1762T/G1764A）突变。在核心区域，四个亚基因型 A1 序列中鉴定出 G1888A 突变。总之，本研究通过完整的 HBV 基因组分析，在 HBV/HIV 共感染和 HBV 单感染患者中增加了南非有限的 HBV 基因型和突变数据。亚基因型 A1 占优势，研究中未检测到耐药突变。J. Med. Virol. 88:1560-1566, 2016。 © 2016 Wiley Periodicals, Inc.
