State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Bioresour Technol. 2016 May;207:213-9. doi: 10.1016/j.biortech.2016.02.009. Epub 2016 Feb 6.
Ferric oxide nanoparticles (FONPs) were used to facilitate dark hydrogen fermentation using Enterobacter aerogenes. The hydrogen yield of glucose increased from 164.5±2.29 to 192.4±1.14mL/g when FONPs concentration increased from 0 to 200mg/L. SEM images of E. aerogenes demonstrated the existence of bacterial nanowire among cells, suggesting FONPs served as electron conduits to enhance electron transfer. TEM showed cellular internalization of FONPs, indicating hydrogenase synthesis and activity was potentially promoted due to the released iron element. When further increasing FONPs concentration to 400mg/L, the hydrogen yield of glucose decreased to 147.2±2.54mL/g. Soluble metabolic products revealed FONPs enhanced acetate pathway of hydrogen production, but weakened ethanol pathway. This shift of metabolic pathways allowed more nicotinamide adenine dinucleotide for reducing proton to hydrogen.
氧化铁纳米颗粒 (FONPs) 被用于促进肠杆菌属 Aerogenes 进行暗发酵产氢。当 FONPs 浓度从 0 增加到 200mg/L 时，葡萄糖的氢气产量从 164.5±2.29mL/g 增加到 192.4±1.14mL/g。肠杆菌属 Aerogenes 的 SEM 图像显示了细胞间存在细菌纳米线，表明 FONPs 充当电子导体以增强电子转移。TEM 显示了 FONPs 的细胞内化，表明由于释放的铁元素，氢化酶的合成和活性可能得到了促进。当进一步将 FONPs 浓度增加到 400mg/L 时，葡萄糖的氢气产量下降到 147.2±2.54mL/g。可溶性代谢产物表明 FONPs 增强了产氢的乙酸途径，但削弱了乙醇途径。这种代谢途径的转变使得更多的烟酰胺腺嘌呤二核苷酸用于将质子还原为氢气。
