State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Bioresour Technol. 2017 Mar;227:50-55. doi: 10.1016/j.biortech.2016.12.033. Epub 2016 Dec 16.
Nuclear irradiation was used for the first time to generate efficient mutants of hydrogen-producing bacteria Enterobacter aerogenes, which were screened with larger colour circles of more fermentative acid by-products. E. aerogenes cells were mutated by nuclear irradiation of Co γ-rays. The screened E. aerogenes ZJU1 mutant with larger colour circles enhanced the hydrogenase activity from 89.8 of the wild strain to 157.4mLH/(gDWh). The hereditary stability of the E. aerogenes ZJU1 mutant was certified after over ten generations of cultivation. The hydrogen yield of 301mLH/gglucose with the mutant was higher by 81.8% than that of 166mL/gglucose with the wild strain. The peak hydrogen production rate of 27.2mL/(L·h) with the mutant was higher by 40.9% compared with that of 19.3mL/(L·h) with the wild strain. The mutant produced more acetate and butyrate but less ethanol compared with the wild strain during hydrogen fermentation.
首次利用核辐照技术选育出产氢效率更高的大肠埃希氏菌突变株，该突变株发酵副产物产酸更多，生成更大的颜色圈。采用 Coγ射线对大肠埃希氏菌进行核辐照诱变，筛选出产氢酶活性由野生型菌株的 89.8提高到 157.4mLH/（gDWh）的大肠埃希氏菌 ZJU1 突变株。经过十多代培养，证明了大肠埃希氏菌 ZJU1 突变株的遗传稳定性。突变株的氢气产量为 301mLH/gglucose，比野生型菌株的 166mL/gglucose 提高了 81.8%。突变株的最大比产氢速率为 27.2mL/（L·h），比野生型菌株的 19.3mL/（L·h）提高了 40.9%。与野生型菌株相比，突变株在产氢发酵过程中产生更多的乙酸和丁酸，但产生的乙醇较少。
