Etienne Kizee A, Roe Chandler C, Smith Rachel M, Vallabhaneni Snigdha, Duarte Carolina, Escadon Patricia, Castaneda Elizabeth, Gomez Beatriz L, de Bedout Catalina, López Luisa F, Salas Valentina, Hederra Luz Maria, Fernandez Jorge, Pidal Paola, Hormazabel Juan Carlos, Otaiza Fernando, Vannberg Fredrik O, Gillece John, Lemmer Darrin, Driebe Elizabeth M, Englethaler David M, Litvintseva Anastasia P
Emerg Infect Dis. 2016 Mar;22(3):476-81. doi: 10.3201/eid2203.151193.
We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (<5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures.
我们使用全基因组序列分型（WGST）来调查2013年至2014年期间在哥伦比亚和智利的肿瘤患者中与接受受污染的抗恶心药物相关的基利安萨罗菌血流感染（BSI）暴发。对25株暴发分离株（18株来自患者，7株来自药瓶）和11株与此次暴发无关的对照分离株进行WGST，以阐明感染源。所有暴发分离株几乎无法区分（<5个单核苷酸多态性），而从无关对照分离株中鉴定出>21,000个单核苷酸多态性，表明此次暴发为点源暴发。基利安萨罗菌此前已被认为与医疗相关感染有关；然而，缺乏可用的分型方法使得无法证实感染源。对于由没有参考基因组或现有基因分型方法的真核病原体引起的暴发进行WGST，能够准确识别感染源，以指导实施适当的控制和预防措施。
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