Ganguli Arnab, Das Amlan, Nag Debasish, Bhattacharya Surela, Chakrabarti Gopal
Department of Biotechnology and Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, WB, 700019, India.
Department of Biotechnology and Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, WB, 700019, India.
Food Chem Toxicol. 2016 Apr;90:160-70. doi: 10.1016/j.fct.2016.02.011. Epub 2016 Feb 15.
Toxic components of STE induced serious, adverse human oral health outcomes. In the present study, we observed that STE was involved in oral toxicity by reducing the viability of human squamous epithelial cells, SCC-25, along with the simultaneous induction of both apoptosis and autophagic signaling. STE was also found to induce significant amount ROS generation in SCC-25 cells. The dietary flavonoid morin, found abundantly in a variety of herbs, fruits and wine, has been reported to attenuate ROS-induced pathogenesis including autophagy. In this study we designed three different treatment regimes of morin treatment, such as pre, co, and post - treatment of STE challenged SCC-25 cells. In all cases morin provided cytoprotection to STE challenged SCC-25 cells by augmenting STE induced ROS-dependent cytotoxic autophagy. Hence, morin is a potential option for antioxidant therapy in treatment of STE induced toxicity.
槟榔的毒性成分会导致严重的不良口腔健康后果。在本研究中，我们观察到槟榔通过降低人鳞状上皮细胞SCC - 25的活力以及同时诱导凋亡和自噬信号传导而参与口腔毒性作用。还发现槟榔会在SCC - 25细胞中诱导大量活性氧生成。在各种草药、水果和葡萄酒中大量存在的膳食类黄酮桑色素，据报道可减轻包括自噬在内的活性氧诱导的发病机制。在本研究中，我们设计了三种不同的桑色素处理方案，即对槟榔刺激的SCC - 25细胞进行预处理、共处理和后处理。在所有情况下，桑色素通过增强槟榔诱导的活性氧依赖性细胞毒性自噬，为槟榔刺激的SCC - 25细胞提供细胞保护作用。因此，桑色素是治疗槟榔诱导毒性的抗氧化治疗的潜在选择。
