Satyanarayana K, Rao M R
Department of Biochemistry, Indian Institute of Science, Bangalore.
Exp Cell Res. 1989 Dec;185(2):319-26. doi: 10.1016/0014-4827(89)90302-9.
Rat testicular nuclei have been probed for the presence of Z-DNA conformation by employing indirect immunofluorescence technique using anti-Z-DNA antibodies. Pachytene nuclei, in which meiotic recombination takes place, showed brighter fluorescence than the premeiotic and postmeiotic spermatogenic nuclei. Moreover, utilizing a novel chromatin immunoblotting technique, Z-DNA conformation was found to be enriched in the poly(ADP-ribosyl)ated chromatin domains of the pachytene nucleus.
