用废甘油培养普通小球藻：提高营养物质去除率和增加油脂产量的策略。
Cultivation of Chlorella vulgaris in wastewater with waste glycerol: Strategies for improving nutrients removal and enhancing lipid production.
机构信息
Institute of Microbiology, Chinese Academy of Sciences, A3 Datun Rd., Chaoyang District, Beijing 100101, People's Republic of China; Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Avenue, St. Paul, MN 55108, United States.
Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Avenue, St. Paul, MN 55108, United States; MOE Biomass Energy Research Center and College of Food Science and State Key Laboratory of Food Science and Technology, Nanchang University, Jiangxi 330047, People's Republic of China.
出版信息
Bioresour Technol. 2016 May;207:252-61. doi: 10.1016/j.biortech.2016.02.013. Epub 2016 Feb 9.
To improve nutrients removal from wastewater and enhance lipid production, cultivation of Chlorella vulgaris in wastewater with waste glycerol generated from biodiesel production using scum derived oil as feedstock was studied. The results showed that nutrients removal was improved and lipid production of C. vulgaris was enhanced with the addition of waste glycerol into wastewater to balance its C/N ratio. The optimal concentration of the pretreated glycerol for C. vulgaris was 10gL(-1) with biomass concentration of 2.92gL(-1), lipid productivity of 163mgL(-1)d(-1), and the removal of 100% ammonia and 95% of total nitrogen. Alkaline conditions prompted cell growth and lipid accumulation of C. vulgaris while stimulating nutrients removal. The application of the integration process can lower both wastewater treatment and biofuel feedstock costs.
为提高废水中营养物质的去除率并促进油脂生成，以浮渣油为原料生物柴油生产过程中产生的废甘油为碳源培养普通小球藻。结果表明，废水中添加废甘油可以平衡 C/N 比，从而提高营养物质的去除率，促进普通小球藻油脂的生成。预处理后的甘油最适浓度为 10 g/L，此时普通小球藻的生物量浓度为 2.92 g/L，油脂生产率为 163 mg/L·d-1，氨氮去除率为 100%，总氮去除率为 95%。碱性条件促进了普通小球藻的细胞生长和脂质积累，同时也促进了营养物质的去除。该集成工艺的应用可以降低废水处理和生物燃料原料成本。
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