利用实际沼液和废甘油在中试规模光生物反应器中培养普通小球藻，以提高微藻生物质产量和去除废水中的营养物质。
Cultivation of Chlorella vulgaris in a pilot-scale photobioreactor using real centrate wastewater with waste glycerol for improving microalgae biomass production and wastewater nutrients removal.
机构信息
School of Environmental and Civil Engineering, Jiangnan University, China; Department of Bioproducts and Biosystems Engineering, and Center for Biorefining, University of Minnesota, United States.
School of Environmental and Civil Engineering, Jiangnan University, China.
出版信息
Bioresour Technol. 2017 Dec;245(Pt A):1130-1138. doi: 10.1016/j.biortech.2017.09.040. Epub 2017 Sep 8.
To improve nutrients removal from real centrate wastewater and enhance the microalgae biomass production, cultivation of Chlorella vulgaris in lab and a pilot-scale photobioreactor with waste glycerol was studied. The results showed the optimal concentration of the crude glycerol was 1.0gL with the maximum biomass productivity of 460mgLd TVS, the maximum lipid content of 27%, the nutrient removal efficiency of all above 86%, due to more balanced C/N ratio. The synergistic relationship between the wastewater-borne bacteria and the microalgae had significant good influence on nutrient removal. In pilot-scale wastewater-based algae cultivation, with 1gL waste glycerol addition, the average biomass production of 16.7gmd, lipid content of 23.6%, and the removal of 2.4gmd NH-N, 2.7gmd total nitrogen, 3.0gmd total phosphorous, and 103.0gmd of COD were attained for 34days semi-continuous mode.
为了提高实际浓缩废水中营养物质的去除率并提高微藻生物质的产量，研究了在实验室和使用废甘油的中试规模光生物反应器中培养普通小球藻。结果表明，粗甘油的最佳浓度为 1.0g/L，最大生物质生产率为 460mg/L/dTVS，最大脂质含量为 27%，由于更平衡的 C/N 比，所有营养物质的去除效率均超过 86%。废水中的细菌和微藻之间的协同关系对营养物质的去除有显著的良好影响。在中试规模的基于废水的藻类培养中，添加 1g/L 废甘油，在 34 天的半连续模式下，平均生物质产量为 16.7g/md，脂质含量为 23.6%，去除 2.4g/md NH-N、2.7g/md 总氮、3.0g/md 总磷和 103.0g/md COD。
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