乙基磺酸甲酯诱变选育高产生物质和油脂的杜氏藻。
Breeding of high biomass and lipid producing Desmodesmus sp. by Ethylmethane sulfonate-induced mutation.
机构信息
Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
出版信息
Bioresour Technol. 2016 May;207:268-75. doi: 10.1016/j.biortech.2016.01.120. Epub 2016 Feb 16.
To improve the biomass yield and lipid productivity, two desert microalgae, Desmodesmus sp. S81 and G41 were induced mutagenesis by Ethylmethane sulfonate (EMS), and obtained two potential mutants, Desmodesmus sp. S5 and G3 from the mutagenic clones for their greatly promoted biomass and lipid production. The results showed that the biomass yield, lipid content and lipid productivity of the mutant strains S5 and G3 were 778.10mg·L(-1), 48.41% and 19.83mg·L(-1)·d(-1), 739.52mg·L(-1), 46.01%, and 17.92mg·L(-1)·d(-1), respectively, which presented the increment of 45.50%, 8.00% and 74.24%, 20.67%, 10.35% and 55.77% than those of S81 and G41. Comparing with the wild strains, the mutants showed reduced PUFAs and glycol lipids, elevated MUFAs and neutral lipids contents, which were appropriate for biodiesel production.
为提高生物质产量和油脂生产力，采用乙基磺酸甲酯（EMS）对两种沙漠微藻——斯氏杜氏藻 S81 和 G41 进行诱变处理，从诱变克隆中获得了两个具有显著促进生物质和油脂生产能力的潜在突变体 S5 和 G3。结果表明，突变株 S5 和 G3 的生物质产量、油脂含量和油脂生产力分别为 778.10mg·L(-1)、48.41%和 19.83mg·L(-1)·d(-1)、739.52mg·L(-1)、46.01%和 17.92mg·L(-1)·d(-1)，与 S81 和 G41 相比，分别提高了 45.50%、8.00%和 74.24%、20.67%、10.35%和 55.77%。与野生株相比，突变株的多不饱和脂肪酸和糖脂含量降低，单不饱和脂肪酸和中性脂含量升高，适合生物柴油生产。
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