Xu Qingshan, Wang Yu, Ding Zhaotang, Song Lubin, Li Yusheng, Ma Dexin, Wang Yi, Shen Jiazhi, Jia Sisi, Sun Haiwei, Zhang Hong
College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China; State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China.
College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China.
Plant Physiol Biochem. 2016 Apr;101:162-172. doi: 10.1016/j.plaphy.2016.02.001. Epub 2016 Feb 5.
Tea [Camellia sinensis (L.)], is an aluminum (Al(3+)) hyperaccumulator plant and grows well in acid soils. In the present study, roots of two tea cultivars, JHC and YS were treated with different concentrations of Al(3+). After treatments, the root length, dry matter, root activity and chlorophyll content (SPAD value) of JHC had greater increase than that of YS. We also detected metabolic changes of two varieties using GC-MS method. Comparison between two cultivars indicated that shikimic pathway was more enhanced in YS roots by Al(3+) with higher levels of catechine, quinic acid and shikimic acid. While, more active amino acid synthesis was found in JHC roots and JHC leaves remained the higher level contents of metabolites related to cysteine synthesis. The comparison also showed that a large amount of sugar alcohols were accumulated in roots of two varieties, whereas most of them were reduced in YS leaves. Other well-known ligands, such as phosphoric acid and malic acid were observed in two cultivars that showed significantly altered abundances under Al(3+) treatments. The results indicated that Al(3+) adaptation of two cultivars may be correlated with their differential metabolism of amino acids, sugars and shikimic acids.
茶树[Camellia sinensis (L.)]是一种铝（Al(3+)）超积累植物，在酸性土壤中生长良好。在本研究中，对两个茶树品种金观音（JHC）和迎霜（YS）的根系进行了不同浓度Al(3+)处理。处理后，金观音的根长、干物质、根系活力和叶绿素含量（SPAD值）的增加幅度大于迎霜。我们还使用气相色谱-质谱联用（GC-MS）方法检测了两个品种的代谢变化。两个品种之间的比较表明，Al(3+)处理使迎霜根系中的莽草酸途径增强，儿茶素、奎尼酸和莽草酸水平更高。而金观音根系中的氨基酸合成更活跃，金观音叶片中与半胱氨酸合成相关的代谢物含量保持在较高水平。比较还表明，两个品种的根系中都积累了大量糖醇，而迎霜叶片中的大部分糖醇含量降低。在两个品种中还观察到其他知名配体，如磷酸和苹果酸，它们在Al(3+)处理下丰度显著改变。结果表明，两个品种对Al(3+)的适应性可能与其氨基酸、糖类和莽草酸的差异代谢有关。
