通过链霉菌属中脂酶素产生菌的培养基工程化调节脂肪酸代谢和三羧酸循环来提高脂酶素的产量。

Modulation of fatty acid metabolism and tricarboxylic acid cycle to enhance the lipstatin production through medium engineering in Streptomyces toxytricini.

机构信息

Microbial Biotechnology Laboratory, University Institute of Engineering and Technology, Maharshi Dayanand University, Rohtak, Haryana, India.

出版信息

Bioresour Technol. 2016 Aug;213:64-68. doi: 10.1016/j.biortech.2016.01.133. Epub 2016 Feb 11.

DOI:10.1016/j.biortech.2016.01.133

PMID:26897471

Abstract

This work investigated the potential of medium engineering to obtain maximum biomass, non-conventional carbon sources for lipstatin production and modulation of tricarboxylic acid (TCA) cycle to promote lipstatin synthesis. It was found that 2:3 carbon and nitrogen ratio, produced maximum biomass of 7.9g/L in growth medium and 6.6g/L in pre-seed medium. Among the studied non-conventional carbon sources i.e., soya flour 40g/L and sesame oil 30mL/L were found producing 1109.37mg/L (1.24-fold of control) and 1196.75mg/L (1.34-fold of control) lipstatin respectively. Supplementation of TCA cycle intermediates revealed that NADH and succinic acid showed lipstatin production to 1132.99mg/L and 1171.10mg/L respectively. Experimental outcome was validated in 7L bioreactor and produced 2242.63mg/L lipstatin which was ∼14% higher than shake flask.

摘要

本研究旨在通过培养基工程优化，获得最大生物量、非常规碳源用于脂肪酶生产，并调节三羧酸（TCA）循环以促进脂肪酶合成。结果表明，在生长培养基和预种培养基中，碳氮比为 2:3 时可分别获得最大生物量 7.9g/L 和 6.6g/L。在所研究的非常规碳源中，豆粉 40g/L 和芝麻油 30mL/L 分别产生 1109.37mg/L（对照的 1.24 倍）和 1196.75mg/L（对照的 1.34 倍）脂肪酶。TCA 循环中间产物的添加表明，NADH 和琥珀酸分别使脂肪酶产量达到 1132.99mg/L 和 1171.10mg/L。在 7L 生物反应器中的实验结果表明，脂肪酶产量为 2242.63mg/L，比摇瓶提高了约 14%。