通过优化发酵条件和培养基提高毒三素链霉菌产脂抑素的产量。

Enhanced production of lipstatin from Streptomyces toxytricini by optimizing fermentation conditions and medium.

作者信息

Zhu Tingheng, Wang Lingfei, Wang Weixia, Hu Zhongce, Yu Meilan, Wang Kun, Cui Zhifeng

机构信息

College of Biological and Environmental Engineering, Zhejiang University of Technology.

出版信息

J Gen Appl Microbiol. 2014;60(3):106-11. doi: 10.2323/jgam.60.106.

DOI:10.2323/jgam.60.106

PMID:25008166

Abstract

This paper is concerned with optimization of fermentation conditions for lipstatin production with Streptomyces toxytricini zjut011 by the single factor and orthogonal tests. Five single factors of important effects on lipstatin production were explored. L-Leucine was identified to be the most suitable precursor for lipstatin biosynthesis and for the first time the divalent cations Mg(2+), Co(2+) and Zn(2+) were found to have significant effect on enhancing lipstatin fermentation titer. The effects of the additives on the lipstatin production were in the order of L-leucine ＞ Mg(2+) ＞ Co(2+) ＞ Zn(2+) ＞ octanoic acid. The optimized conditions for lipstatin production were determined as 45.72 mmol/L of L-leucine (added on the 4 th day), 31.1985 mmol/L of octanoic acid (added on the 6th day), 12 mmol/L of Mg(2+), 1 mmol/L of Co(2+) and 0.25 mmol/L of Zn(2+). Under these conditions, a maximum lipstatin of 4.208 g/ml was achieved in verification experiments in 500 ml shake flasks.

摘要

本文通过单因素试验和正交试验，对毒三素链霉菌zjut011产脂肽素的发酵条件进行优化。探讨了对脂肽素产量有重要影响的5个单因素。确定L-亮氨酸是脂肽素生物合成最合适的前体，并且首次发现二价阳离子Mg(2+)、Co(2+)和Zn(2+)对提高脂肽素发酵效价有显著影响。添加剂对脂肽素产量的影响顺序为：L-亮氨酸＞Mg(2+)＞Co(2+)＞Zn(2+)＞辛酸。确定脂肽素生产的优化条件为：45.72 mmol/L L-亮氨酸（第4天添加）、31.1985 mmol/L辛酸（第6天添加）、12 mmol/L Mg(2+)、1 mmol/L Co(2+)和0.25 mmol/L Zn(2+)。在此条件下，500 ml摇瓶的验证实验中脂肽素最高产量达到4.208 g/ml。