Laboratory of Food Chemistry, Wageningen University , Wageningen, The Netherlands.
Faculty of Veterinary Medicine, Institute for Risk Assessment Sciences, Subdivision of Veterinary Pharmacology, Pharmacotherapy, and Toxicology, Utrecht University , Utrecht, The Netherlands.
J Agric Food Chem. 2016 Mar 16;64(10):2127-33. doi: 10.1021/acs.jafc.5b05384. Epub 2016 Mar 1.
In this study, the in vitro fermentation by piglet fecal inoculum of galacto-oligosaccharides (GOS) and porcine milk oligosaccharides (PMOs) was investigated to identify possible preferences for individual oligosaccharide structures by piglet microbiota. First, acidic PMOs and GOS with degrees of polymerization 4-7 were depleted within 12 h of fermentation, whereas fucosylated and phosphorylated PMOs were partially resistant to fermentation. GOS structures containing β1-3 and β1-2 linkages were preferably fermented over GOS containing β1-4 and β1-6 linkages. Upon in vitro fermentation, acetate and butyrate were produced as the main organic acids. GOS fermentation by piglet inoculum showed a unique fermentation pattern with respect to preference of GOS size and organic acids production.
本研究通过仔猪粪便接种物对半乳糖寡糖（GOS）和猪乳寡糖（PMOs）进行体外发酵，以鉴定仔猪肠道微生物对不同寡糖结构的可能偏好。首先，在发酵 12 小时内，聚合度为 4-7 的酸性 PMOs 和 GOS 被消耗殆尽，而带有岩藻糖基和磷酸基的 PMOs 部分抵抗发酵。含有β1-3 和β1-2 键的 GOS 结构比含有β1-4 和β1-6 键的 GOS 更易发酵。体外发酵时，产生乙酸和丁酸作为主要有机酸。仔猪接种物发酵 GOS 表现出独特的发酵模式，对 GOS 大小和有机酸生成有偏好。
