Nemny-Lavy E, Nestel D, Rempoulakis P
Department of Entomology,Institute of Plant Protection,The Volcani Center,ARO,P.O. Box 6,Beit-Dagan 50250,Israel.
Bull Entomol Res. 2016 Jun;106(3):415-9. doi: 10.1017/S0007485316000092. Epub 2016 Feb 22.
The effect of a sub-sterilizing gamma radiation dose on Dacus ciliatus adults was investigated to assess the suitability of the sterile insect technique (SIT) as an alternative method to control this pest. Late pupae (48 h prior to adult emergence) from a laboratory strain were irradiated with 120 Gy of gamma rays emitted by a 60Co source. Following adult emergence, the mortality of irradiated and non-irradiated cohorts was recorded. Over a period of 50 days after emergence, no significant negative effects of irradiation upon the longevity of male or female laboratory flies were observed. A laboratory competitiveness study (Fried test), using irradiated laboratory and wild males at a ratio of 3:1 was conducted to assess the ability of irradiated males to reduce the egg hatch rates of a wild population. The overall competitiveness was found to be ca. 0.32, suggesting a reduced, but satisfactory, quality of irradiated laboratory as compared with wild males. Based on the above findings, we calculated and proposed effective male release ratios for field application of SIT against D. ciliatus.
研究了亚不育剂量的伽马辐射对桔小实蝇成虫的影响，以评估不育昆虫技术（SIT）作为控制这种害虫的替代方法的适用性。用60Co源发出的120 Gy伽马射线辐照实验室品系的晚期蛹（成虫羽化前48小时）。成虫羽化后，记录辐照和未辐照群体的死亡率。在羽化后的50天内，未观察到辐照对实验室雌雄蝇寿命有显著负面影响。进行了一项实验室竞争力研究（弗里德试验），以3:1的比例使用辐照的实验室雄蝇和野生雄蝇，评估辐照雄蝇降低野生种群卵孵化率的能力。总体竞争力约为0.32，表明与野生雄蝇相比，辐照实验室雄蝇的质量有所下降，但仍令人满意。基于上述发现，我们计算并提出了在田间应用SIT防治桔小实蝇时有效的雄蝇释放比率。
