性信息素二十七烷可增强不育埃及伊蚊雄蚊的交配竞争力。

The sex pheromone heptacosane enhances the mating competitiveness of sterile Aedes aegypti males.

机构信息

The Key Laboratory of Microbiology and Parasitology of Anhui Province, the Key Laboratory of Zoonoses of High Institutions in Anhui, Department of Pathogen Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China.

Department of Radiotherapy, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

出版信息

Parasit Vectors. 2023 Mar 15;16(1):102. doi: 10.1186/s13071-023-05711-6.

DOI:10.1186/s13071-023-05711-6

PMID:36922826

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10015913/

Abstract

BACKGROUND

Aedes aegypti is a vector that transmits various viral diseases, including dengue and Zika. The radiation-based sterile insect technique (SIT) has a limited effect on mosquito control because of the difficulty in irradiating males without reducing their mating competitiveness. In this study, the insect sex pheromone heptacosane was applied to Ae. aegypti males to investigate whether it could enhance the mating competitiveness of irradiated males.

METHODS

Heptacosane was smeared on the abdomens of Ae. aegypti males that were allowed to mate with untreated virgin females. The insemination rate was used to assess the attractiveness of heptacosane-treated males to females. The pupae were irradiated with different doses of X-rays and γ-rays, and the emergence, survival time, egg number, and hatch rate were detected to find the optimal dose of X-ray and γ-ray radiation. The males irradiated at the optimal dose were smeared with heptacosane, released in different ratios with untreated males, and mated with females. The effect of heptacosane on the mating competitiveness of irradiated mosquitoes was then evaluated by the hatch rate, induced sterility, and mating competitiveness index.

RESULTS

Applying heptacosane to Ae. aegypti males significantly increased the insemination rate of females by 20%. Pupal radiation did not affect egg number but significantly reduced survival time and hatch rate. The emergence of the pupae was not affected by X-ray radiation but was affected by γ-ray radiation. Pupae exposed to 60 Gy X-rays and 40 Gy γ-rays were selected for subsequent experiments. After 60 Gy X-ray irradiation or 40 Gy γ-ray irradiation, the average hatch rate was less than 0.1%, and the average survival time was more than 15 days. Moreover, at the same release ratio, the hatch rate of the irradiated group perfumed with heptacosane was lower than that of the group without heptacosane. Conversely, the male sterility and male mating competitiveness index were significantly increased due to the use of heptacosane.

CONCLUSIONS

The sex pheromone heptacosane enhanced the interaction between Ae. aegypti males and females. Perfuming males irradiated by X-rays or γ-rays with heptacosane led to a significant increase in mating competitiveness. This study provided a new idea for improving the application effect of SIT.

摘要

背景

埃及伊蚊是一种传播登革热和寨卡病毒等多种病毒病的媒介。基于辐射的不育昆虫技术（SIT）对控制蚊子的效果有限，因为难以在不降低雄性交配竞争力的情况下对其进行辐照。在这项研究中，应用昆虫性信息素二十七烷来研究它是否可以增强辐照雄性的交配竞争力。

方法

将二十七烷涂抹在与未处理的处女雌性交配的埃及伊蚊雄性的腹部上。用授精率来评估涂有二十七烷的雄性对雌性的吸引力。用不同剂量的 X 射线和γ射线辐照蛹，检测羽化率、存活时间、产卵数和孵化率，以找到 X 射线和γ射线辐照的最佳剂量。用最佳剂量的 X 射线和γ射线辐照雄性，然后涂抹二十七烷，并以不同比例与未处理的雄性一起释放，与雌性交配。然后通过孵化率、诱导不育和交配竞争力指数来评估二十七烷对辐照蚊子交配竞争力的影响。

结果

将二十七烷应用于埃及伊蚊雄性，可使雌性的授精率显著提高 20%。蛹的辐射处理不影响产卵数，但显著降低了存活时间和孵化率。X 射线辐照对蛹的羽化率没有影响，但γ射线辐照有影响。选择暴露于 60Gy X 射线和 40Gy γ射线的蛹进行后续实验。经 60Gy X 射线或 40Gy γ 射线照射后，平均孵化率低于 0.1%，平均存活时间超过 15 天。此外，在相同的释放比例下，涂有二十七烷的辐照组的孵化率低于未涂有二十七烷的组。相反，由于使用了二十七烷，雄性不育率和雄性交配竞争力指数显著增加。