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蓖麻毒素A链中谷氨酸177在核糖体酶促失活中的作用。

Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes.

作者信息

Schlossman D, Withers D, Welsh P, Alexander A, Robertus J, Frankel A

机构信息

Department of Medicine, Duke University Medical Center, Durham, North Carolina 22710.

出版信息

Mol Cell Biol. 1989 Nov;9(11):5012-21. doi: 10.1128/mcb.9.11.5012-5021.1989.

DOI:10.1128/mcb.9.11.5012-5021.1989
PMID:2689871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363653/
Abstract

The gene for the A chain of ricin toxin was fused to a beta-galactosidase marker cistron via a DNA sequence encoding a short collagen linker, and the tripartite fusion protein was expressed in Escherichia coli. Site-specific mutagenesis was used to change glutamic acid residue 177 to aspartic acid or alanine. When the mutant proteins were expressed, purified, and tested quantitatively for enzymatic activity, the carboxylate function at position 177 was found not to be absolutely essential for ricin toxin A-chain catalysis.

摘要

蓖麻毒素A链基因通过编码短胶原连接子的DNA序列与β-半乳糖苷酶标记顺反子融合，并且该三方融合蛋白在大肠杆菌中表达。利用定点诱变将谷氨酸残基177变为天冬氨酸或丙氨酸。当表达、纯化突变蛋白并对其酶活性进行定量检测时，发现177位的羧基功能对于蓖麻毒素A链催化并非绝对必要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/413d/363653/99353b22c073/molcellb00059-0441-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/413d/363653/746754c211eb/molcellb00059-0438-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/413d/363653/99353b22c073/molcellb00059-0441-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/413d/363653/746754c211eb/molcellb00059-0438-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/413d/363653/99353b22c073/molcellb00059-0441-a.jpg

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1
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Exp Cell Res. 1980 Apr;126(2):321-6. doi: 10.1016/0014-4827(80)90270-0.
2
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.
3
Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K.
Pokeweed antiviral protein: its cytotoxicity mechanism and applications in plant disease resistance.
商陆抗病毒蛋白：其细胞毒性机制及在植物抗病性中的应用
Toxins (Basel). 2015 Mar 6;7(3):755-72. doi: 10.3390/toxins7030755.
4
Wild type RTA and less toxic variants have distinct requirements for Png1 for their depurination activity and toxicity in Saccharomyces cerevisiae.野生型RTA和毒性较低的变体在酿酒酵母中进行脱嘌呤活性和毒性时，对Png1有不同的要求。
PLoS One. 2014 Dec 1;9(12):e113719. doi: 10.1371/journal.pone.0113719. eCollection 2014.
5
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J Biol Chem. 2013 Oct 18;288(42):30270-30284. doi: 10.1074/jbc.M113.510966. Epub 2013 Sep 3.
6
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Toxicon. 2013 Jul;69:143-51. doi: 10.1016/j.toxicon.2013.02.001. Epub 2013 Feb 20.
7
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8
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9
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Proc Natl Acad Sci U S A. 1983 Nov;80(22):6848-52. doi: 10.1073/pnas.80.22.6848.
4
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Anal Biochem. 1981 Jun;114(1):193-7. doi: 10.1016/0003-2697(81)90473-5.
5
Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.调控酿酒酵母中双向GAL1 - GAL10启动子的序列。
Mol Cell Biol. 1984 Aug;4(8):1440-8. doi: 10.1128/mcb.4.8.1440-1448.1984.
6
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Proc Natl Acad Sci U S A. 1984 Aug;81(15):4692-6. doi: 10.1073/pnas.81.15.4692.
7
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
8
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FEBS Lett. 1972 Nov 15;28(1):48-50. doi: 10.1016/0014-5793(72)80674-4.
9
Induction of galactokinase in Saccharomyces cerevisiae: kinetics of induction and glucose effects.酿酒酵母中半乳糖激酶的诱导：诱导动力学及葡萄糖的影响
J Bacteriol. 1972 Aug;111(2):308-15. doi: 10.1128/jb.111.2.308-315.1972.
10
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J Biol Chem. 1974 Oct 10;249(19):6181-90.