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来自互补组E的着色性干皮病细胞缺乏酵母光裂合酶同源物的证据。

Evidence that xeroderma pigmentosum cells from complementation group E are deficient in a homolog of yeast photolyase.

作者信息

Patterson M, Chu G

机构信息

Department of Medicine, Stanford University School of Medicine, California 94305.

出版信息

Mol Cell Biol. 1989 Nov;9(11):5105-12. doi: 10.1128/mcb.9.11.5105-5112.1989.

DOI:10.1128/mcb.9.11.5105-5112.1989
PMID:2689872
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363662/
Abstract

Xeroderma pigmentosum (XP) patients are deficient in the excision repair of damaged DNA. Recognition of the DNA lesion appears to involve a nuclear factor that is defective in complementation group E (XPE binding factor). We have now identified a factor in the yeast Saccharomyces cerevisiae that shares many properties with XPE binding factor, including cellular location, abundance, magnesium dependence, and relative affinities for multiple forms of damaged DNA. Yeast binding activity is dependent on photolyase, which catalyzes the photoreactivation of pyrimidine dimers. These results suggest that yeast photolyase may also function as an auxiliary protein in excision repair. Furthermore, XPE binding factor appears to be the human homolog of yeast photolyase.

摘要

着色性干皮病（XP）患者在受损DNA的切除修复方面存在缺陷。对DNA损伤的识别似乎涉及一种在互补组E中存在缺陷的核因子（XPE结合因子）。我们现已在酿酒酵母中鉴定出一种与XPE结合因子具有许多共同特性的因子，包括细胞定位、丰度、镁依赖性以及对多种形式受损DNA的相对亲和力。酵母结合活性依赖于光解酶，该酶催化嘧啶二聚体的光复活。这些结果表明，酵母光解酶在切除修复中也可能作为一种辅助蛋白发挥作用。此外，XPE结合因子似乎是酵母光解酶的人类同源物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a367/363662/5212fd12f778/molcellb00059-0533-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a367/363662/928cd5b6d302/molcellb00059-0531-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a367/363662/bbf21aff5829/molcellb00059-0531-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a367/363662/9204ea8e429b/molcellb00059-0532-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a367/363662/3ac4b7f04267/molcellb00059-0533-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a367/363662/5212fd12f778/molcellb00059-0533-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a367/363662/928cd5b6d302/molcellb00059-0531-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a367/363662/bbf21aff5829/molcellb00059-0531-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a367/363662/9204ea8e429b/molcellb00059-0532-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a367/363662/3ac4b7f04267/molcellb00059-0533-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a367/363662/5212fd12f778/molcellb00059-0533-b.jpg

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Lack of DNA enzymatic photoreactivation in HeLa cell-free extracts.
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Xeroderma pigmentosum complementation group E and UV-damaged DNA-binding protein.着色性干皮病E互补组与紫外线损伤DNA结合蛋白

本文引用的文献

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Isolation and characterization of nuclei and nuclear membranes from Saccharomyces cerevisiae protoplasts.从酿酒酵母原生质体中分离并鉴定细胞核和核膜
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Evidence for lack of DNA photoreactivating enzyme in humans.人类缺乏DNA光复活酶的证据。
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Characterization of a DNA-damage-recognition protein from F9 teratocarcinoma cells, which is inducible by retinoic acid and cyclic AMP.来自F9畸胎瘤细胞的一种DNA损伤识别蛋白的特性，该蛋白可被视黄酸和环磷酸腺苷诱导。
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