Orfila C, Rakotoarivony J, Serre G, Rahobisoa N, Suc J M
INSERM U133, CHU Purpan et Laboratoire d'Optique Electronique du CNRS, Toulouse Rangueil, France.
Nephron. 1989;53(4):343-8. doi: 10.1159/000185779.
This preliminary study concerns murine monoclonal antibodies (Mabs) against tubular basement membranes (TBM) of human kidney which were produced by the classical hybridization technique. One of these Mabs (PP8-1) specifically recognized an antigen present in the tubular basement membranes of various portions of tubule and in the Bowman's capsule. By immunocytochemical techniques, this Mab was used to study the presence and the distribution of the related antigen in urinary sediment from 12 normal healthy subjects. This TBM and Bowman's capsule antigen was demonstrated along hyaline urinary cast surface and, in some cases, in free extracellular fibrillar structures morphologically unrelated to the casts. This suggests that, in some normal conditions, catabolism of TBM and/or Bowman's capsule could release material in urine, as it has been described for glomerular basement membrane (GBM) constituents. Such an immunocytochemical approach could be useful to detect components from tubular renal basement membranes in urinary sediment during pathological conditions.
这项初步研究涉及通过经典杂交技术制备的针对人肾小管基底膜（TBM）的鼠单克隆抗体（Mab）。其中一种单克隆抗体（PP8 - 1）特异性识别存在于肾小管各部分的基底膜和鲍曼囊中一种抗原。通过免疫细胞化学技术，该单克隆抗体被用于研究12名正常健康受试者尿沉渣中相关抗原的存在和分布。这种肾小管基底膜和鲍曼囊抗原在透明管型表面被证实存在，在某些情况下，也存在于形态上与管型无关的游离细胞外纤维结构中。这表明，在某些正常情况下，肾小管基底膜和/或鲍曼囊的分解代谢可能会像肾小球基底膜（GBM）成分那样在尿液中释放物质。这种免疫细胞化学方法可能有助于在病理状态下检测尿沉渣中肾小管基底膜的成分。
