Paul L C, Carpenter C B
Kidney Int. 1982 Jun;21(6):800-7. doi: 10.1038/ki.1982.102.
Kidney sections from several inbred rat strains were studied with an indirect immunofluorescence technique using five different, selected rat antisera which contained antibodies against the tubular basement membrane (TBM). The sera came from transplanted animals, an apparently normal animal, and animals treated with spleen cells in complete Freund's adjuvant. Upon panel analysis, at least two different antigenic determinants were recognized on the proximal TBM: One was present in nine and one was present in seven of the 15 strains tested; both determinants appear to be expressed in most antigen-positive strains. Using kidneys from major histocompatibility complex (MHC)-congenic strains, it was shown that the TBM antigens are encoded outside the MHC region. Three sera produced variable staining of Bowman's capsule in association with the proximal TBM; two sera produced clearly discordant staining of Bowman's capsule and proximal TBM. The capsular antigens were also coded outside the MHC region. These observations suggested the existence of at least two different antigenic determinants on proximal TBM. Bowman's capsule appeared to have antigenic determinants in common with the proximal TBM as well as distinct determinants.
使用五种不同的、经过挑选的大鼠抗血清，采用间接免疫荧光技术对几种近交系大鼠品系的肾脏切片进行了研究，这些抗血清含有抗肾小管基底膜（TBM）的抗体。这些血清来自移植动物、一只外观正常的动物以及用完全弗氏佐剂处理过的脾脏细胞的动物。通过分组分析，在近端TBM上识别出至少两种不同的抗原决定簇：在所测试的15个品系中，一种存在于9个品系中，另一种存在于7个品系中；这两种决定簇似乎在大多数抗原阳性品系中都有表达。使用主要组织相容性复合体（MHC）同基因品系的肾脏，结果表明TBM抗原是在MHC区域之外编码的。三种血清与近端TBM相关联地产生了可变的鲍曼囊染色；两种血清产生了明显不一致的鲍曼囊和近端TBM染色。囊抗原也在MHC区域之外编码。这些观察结果表明近端TBM上至少存在两种不同的抗原决定簇。鲍曼囊似乎与近端TBM有共同的抗原决定簇以及独特的决定簇。
